Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Theref...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 21; no. 24; p. 9563
Main Authors:	Santos, Rosana de Almeida, Asensi, Karina Dutra, de Barros, Julia Helena Oliveira, de Menezes, Rafael Campos Silva, Cordeiro, Ingrid Rosenburg, Neto, José Marques de Brito, Kasai-Brunswick, Tais Hanae, Goldenberg, Regina Coeli Dos Santos
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 15-12-2020 MDPI
Subjects:	Angiogenesis Animals Blood circulation Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - metabolism bone marrow mesenchymal stromal cells Cell culture Cell Differentiation Cell Movement Cell Proliferation Cells, Cultured Chick Embryo Disease Embryos Female Fibroblast growth factor 2 Human Umbilical Vein Endothelial Cells Humans Immunohistochemistry Ischemia menstrual blood-derived mesenchymal stromal cells Menstruation Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Mesenchyme Morphology Neovascularization, Physiologic Paracrine signalling Platelet-derived growth factor Spheroids Stromal cells Vascular endothelial growth factor bone marrow mesenchymal stromal cells menstrual blood-derived mesenchymal stromal cells angiogenesis human umbilical vein endothelial cells
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Summary:	Several therapies are being developed to increase blood circulation in ischemic tissues. Despite bone marrow-derived mesenchymal stromal cells (bmMSC) are still the most studied, an interesting and less invasive MSC source is the menstrual blood, which has shown great angiogenic capabilities. Therefore, the aim of this study was to evaluate the angiogenic properties of menstrual blood-derived mesenchymal stromal cells (mbMSC) in vitro and in vivo and compared to bmMSC. MSC's intrinsic angiogenic capacity was assessed by sprouting and migration assays. mbMSC presented higher invasion and longer sprouts in 3D culture. Additionally, both MSC-spheroids showed cells expressing CD31. mbMSC and bmMSC were able to migrate after scratch wound in vitro, nonetheless, only mbMSC demonstrated ability to engraft in the chick embryo, migrating to perivascular, perineural, and chondrogenic regions. In order to study the paracrine effects, mbMSC and bmMSC conditioned mediums were capable of stimulating HUVEC's tube-like formation and migration. Both cells expressed VEGF-A and FGF2. Meanwhile, PDGF-B was expressed exclusively in mbMSC. Our results indicated that mbMSC and bmMSC presented a promising angiogenic potential. However, mbMSC seems to have additional advantages since it can be obtained by non-invasive procedure and expresses PDGF-B, an important molecule for vascular formation and remodeling.
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms21249563