Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile

The effects of bovine leukemia virus (BLV) on the immune response have been extensively investigated; however, its effects on mammary gland immunity are only speculative. Although BLV has a tropism for B cells, it can affect both adaptive and innate immunities because these systems share many effect...

Full description

Saved in:

Bibliographic Details
Published in:	Veterinary research (Paris) Vol. 46; no. 1; p. 2
Main Authors:	Della Libera, Alice Maria Melville Paiva, de Souza, Fernando Nogueira, Batista, Camila Freitas, Santos, Bruna Parapinski, de Azevedo, Luis Fernando Fernandes, Sanchez, Eduardo Milton Ramos, Diniz, Soraia Araújo, Silva, Marcos Xavier, Haddad, João Paulo, Blagitz, Maiara Garcia
Format:	Journal Article
Language:	English
Published:	England Springer-Verlag 2015 BioMed Central Ltd BioMed Central
Subjects:	Animals apoptosis B-Lymphocytes - immunology Bovine leukemia virus Cattle dairy cows Enzootic Bovine Leukosis - immunology Enzootic Bovine Leukosis - pathology Enzootic Bovine Leukosis - virology Female fluorescence immune response Leukemia Virus, Bovine - immunology Life Sciences Lymphocyte Subsets - immunology mammary glands Mammary Glands, Animal - immunology milk Milk - cytology neutrophils Neutrophils - immunology Phagocytosis reactive oxygen species Reactive Oxygen Species - metabolism Staphylococcus aureus Staphylococcus aureus - physiology udder quarters viability
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The effects of bovine leukemia virus (BLV) on the immune response have been extensively investigated; however, its effects on mammary gland immunity are only speculative. Although BLV has a tropism for B cells, it can affect both adaptive and innate immunities because these systems share many effector mechanisms. This scenario is the basis of this investigation of the effects of BLV on mammary gland immunity, which is largely dependent upon neutrophilic functions. Thus, the present study sought to examine neutrophilic functions and the lymphocyte profile in the milk of naturally BLV-infected cows. The viability of the milk neutrophils and the percentage of milk neutrophils that produced reactive oxygen species (ROS) or phagocytosed Staphylococcus aureus were similar between BLV-infected and BLV-uninfected dairy cows. Furthermore, the expression of CD62L and CD11b by the milk neutrophils and the percentage of milk neutrophils (CH138⁺cells) that were obtained from the udder quarters of the BLV-infected cows were not altered. Conversely, the median fluorescence intensity (MFI) representing intracellular ROS production and the phagocytosis of S. aureus, the expression of CD44 by the milk neutrophils and the percentage of apoptotic B cells were lower in the milk cells from BLV-infected dairy cows, particularly those from animals with persistent lymphocytosis (PL). The lymphocyte subsets were not different among the groups, with the exception of the percentage of CD5⁻/CD11b⁻B cells, which was higher in the milk cells from BLV-infected cows, particularly those with PL. Thus, the present study provides novel insight into the implications of BLV infection for mammary gland immunity.
Bibliography:	http://dx.doi.org/10.1186/s13567-014-0125-4 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1297-9716 0928-4249 1297-9716
DOI:	10.1186/s13567-014-0125-4