Immunodiagnosis of human neurocysticercosis using a synthetic peptide selected by phage-display

Abstract The usefulness of a synthetic peptide in the serodiagnosis of Taenia solium human neurocysticercosis (NC) has been evaluated. Phage-displayed peptides were screened with human antibodies to scolex protein antigen from cysticercus cellulosae (SPACc). One clone was found to interact specifica...

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Published in:	Clinical immunology (Orlando, Fla.) Vol. 131; no. 1; pp. 129 - 138
Main Authors:	Hell, R.C.R, Amim, P, de Andrade, H.M, de Avila, R.A.M, Felicori, L, Oliveira, A.G, Oliveira, C.A, Nascimento, E, Tavares, C.A.P, Granier, C, Chávez-Olórtegui, C
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-04-2009 Elsevier
Subjects:	Allergy and Immunology Animals Antibodies, Helminth - blood Antibodies, Helminth - immunology Antibody Specificity Antigens, Helminth - immunology Biological and medical sciences Cysticercosis Diseases caused by cestodes DNA, Helminth - chemistry DNA, Helminth - genetics Electrophoresis, Gel, Two-Dimensional Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Fundamental immunology Helminthic diseases Humans Immunodiagnostic Immunohistochemistry Immunologic Tests - methods Infectious diseases Medical sciences Mice Mice, Inbred BALB C Neurocysticercosis Neurocysticercosis - blood Neurocysticercosis - diagnosis Neurocysticercosis - immunology Neurocysticercosis - parasitology Parasitic diseases Peptide Library Peptides - immunology Phage display Polymerase Chain Reaction Synthetic peptide Taenia Taenia solium Taenia solium - immunology Taenia solium - isolation & purification Phage display Neurocysticercosis Synthetic peptide Immunodiagnostic Human Cysticercosis Peptides Antibody Central nervous system Parasitosis Helminthiasis Cestode disease Infection Immunology Synthetic product Immunological investigation Diagnosis
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Summary:	Abstract The usefulness of a synthetic peptide in the serodiagnosis of Taenia solium human neurocysticercosis (NC) has been evaluated. Phage-displayed peptides were screened with human antibodies to scolex protein antigen from cysticercus cellulosae (SPACc). One clone was found to interact specifically with anti-SPACc IgGs. The corresponding synthetic peptide was found to be recognized in ELISA by NC patient's sera. The study was carried out with sera from 28 confirmed NC patients, 13 control sera and 73 sera from patients suffering from other infectious diseases. A 93% sensibility and a 94.3% specificity was achieved. Figures of 89% and 31.4% of sensibility and specificity were obtained in a SPACc-based ELISA. Immunoblotting of SPACc with anti-peptide antibodies revealed a single band of ∼ 45 kDa in 1D and four 45 kDa isoforms in 2D-gel electrophoresis. A strong and specific immunostaining in the fibers beneath the suckers, at the base of the rostellum, and in the tissue surrounding the scolex of cysticerci was observed by immunomicroscopy. Our results show that a peptide-based immunodiagnostic of neurocisticercosis can be envisioned.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1521-6616 1521-7035
DOI:	10.1016/j.clim.2008.10.012