Apoptosis in canine distemper

Canine distemper is a systemic viral disease characterized by immunosuppression followed by secondary infections. Apoptosis is observed in several immunosuppressive diseases and its occurrence on canine distemper in vivo has not been published. In this study, the occurrence of apoptosis was determin...

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Bibliographic Details
Published in:	Archives of virology Vol. 148; no. 1; pp. 153 - 164
Main Authors:	Moro, L, de Sousa Martins, A, de Moraes Alves, C, de Araújo Santos, F. G, dos Santos Nunes, J. E, Carneiro, R. A, Carvalho, R, Vasconcelos, A. C
Format:	Journal Article
Language:	English
Published:	Wien Springer-Verlag 2003 New York, NY Springer Springer Nature B.V
Subjects:	Animal viral diseases Animals Apoptosis Biological and medical sciences brain Brain - immunology Brain - pathology canine distemper Canine distemper virus Distemper - immunology Distemper - pathology Distemper - virology Distemper Virus, Canine - immunology DNA DNA Fragmentation DNA, Viral - immunology Dogs histopathology immunohistochemistry Infectious diseases lymph nodes Lymph Nodes - immunology Lymph Nodes - pathology Medical sciences morphometry nucleoproteins Nucleoproteins - metabolism puppies secondary infection spleen Spleen - immunology Spleen - pathology thymus gland Thymus Gland - immunology Thymus Gland - pathology tissues Viral diseases Fissipedia Carnivora Pathogenesis Lymphoid tissue Host virus relation Canine distemper Infection Vertebrata Immunosuppression Mammalia Cell death Viral disease Dog Apoptosis
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Summary:	Canine distemper is a systemic viral disease characterized by immunosuppression followed by secondary infections. Apoptosis is observed in several immunosuppressive diseases and its occurrence on canine distemper in vivo has not been published. In this study, the occurrence of apoptosis was determined in lymphoid tissues of thirteen naturally infected dogs and nine experimentally inoculated puppies. Healthy dogs were used as negative controls. Samples of lymph nodes, thymus, spleen and brain were collected for histopathological purposes. Sections, 5 μm thick, of retropharingeal lymph nodes were stained by HE, Shorr, Methyl Green-Pyronin and TUNEL reaction. Shorr stained sections were further evaluated by morphometry. Canine distemper virus nucleoprotein was detected by immunohistochemistry. Retropharingeal lymph nodes of naturally and experimentally infected dogs had more apoptotic cells per field than controls. In addition, DNA from thymus of infected dogs were more fragmented than controls. Therefore, apoptosis is increased in lymphoid depletion induced by canine distemper virus and consequently play a role in the immunosuppression seen in this disease.
Bibliography:	http://dx.doi.org/10.1007/s00705-002-0903-6 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-002-0903-6