Determination of synthetic food dyes in commercial soft drinks by TLC and ion-pair HPLC

•Synthetic food colourings were analyzed in soft drinks.•The colourings food were extracted from soft drinks using C18 SPE.•The colourings food were identified by thin layer chromatography.•The concentration of food colouring in soft drink was determined by HPLC. Synthetic food colourings were analy...

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Bibliographic Details
Published in:	Food chemistry Vol. 157; pp. 193 - 198
Main Authors:	de Andrade, Francisca Ivani, Florindo Guedes, Maria Izabel, Pinto Vieira, Ícaro Gusmão, Pereira Mendes, Francisca Noélia, Salmito Rodrigues, Paula Alves, Costa Maia, Carla Soraya, Marques Ávila, Maria Marlene, de Matos Ribeiro, Luzara
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 15-08-2014 Elsevier
Subjects:	Biological and medical sciences Carbonated Beverages - analysis Chromatography, High Pressure Liquid - methods Chromatography, Thin Layer - methods Coloring Agents - analysis Food Coloring Agents - analysis Food toxicology HPLC Medical sciences Molecular Structure Synthetic food colouring Thin layer chromatography Toxicology Vitaceae Synthetic food colouring Thin layer chromatography HPLC Assay Dyes HPLC chromatography Soft drink Food Quantitative analysis
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Summary:	•Synthetic food colourings were analyzed in soft drinks.•The colourings food were extracted from soft drinks using C18 SPE.•The colourings food were identified by thin layer chromatography.•The concentration of food colouring in soft drink was determined by HPLC. Synthetic food colourings were analyzed on commercial carbonated orange and grape soft drinks produced in Ceará State, Brazil. Tartrazine (E102), Amaranth (E123), Sunset Yellow (E110) and Brilliant Blue (E133) were extracted from soft drinks using C18 SPE and identified by thin layer chromatography (TLC), this method was used to confirm the composition of food colouring in soft drinks stated on label. The concentration of food colouring in soft drink was determined by ion-pair high performance liquid chromatography with photodiode array detection. The results obtained with the samples confirm that the identification and quantification methods are recommended for quality control of the synthetic colours in soft drinks, as well as to determine whether the levels and lables complies with the recommendations of food dyes legislation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0308-8146 1873-7072
DOI:	10.1016/j.foodchem.2014.01.100