Influence of Passive Ultrasonic Irrigation of the Photosensitizer Used in Photodynamic Therapy on Microbial Reduction in the Root Canal System: An in Vitro Study

Influence of Passive Ultrasonic Irrigation of the Photosensitizer Used in Photodynamic Therapy on Microbial Reduction in the Root Canal System: An in Vitro Study

The success of endodontic treatment is based on microbial reduction promoted by the interaction of chemical and mechanical procedures. Photodynamic therapy (PDT) is used as an adjunct to conventional treatment, with significant microbial reduction. This study aimed to evaluate the influence of passi...

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Bibliographic Details
Published in:Iranian endodontic journal Vol. 19; no. 2; pp. 105 - 111
Main Authors: de Almeida, Danuza Santos Silvestre, De Martin, Alexandre Sigrist, Pedro Rocha, Daniel Guimarães, Fontana, Carlos Eduardo, Pelegrine, Rina Andréia, da Silveira Bueno, Pelegrine, Pinheiro, Sérgio Luiz
Format: Journal Article
Language:English
Published: Iran Iranian Center for Endodontic Research 01-01-2024
Subjects:
Original
Methylene Blue
Endodontics
Photosensitizing Agents
Photochemotherapy
Root Canal Irrigants
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Summary:The success of endodontic treatment is based on microbial reduction promoted by the interaction of chemical and mechanical procedures. Photodynamic therapy (PDT) is used as an adjunct to conventional treatment, with significant microbial reduction. This study aimed to evaluate the influence of passive ultrasonic irrigation (PUI) of the photosensitizer (PS) used in PDT on microbial reduction in the root canal system. Forty-five mesiobuccal root canals from extracted human mandibular molars that were inoculated with standard strains of , , and for 21 days were selected. The root canals were prepared using the ProTaper Next system and randomly divided into 3 groups ( =15): 1) PDT: PDT alone; 2) PUI+PDT: PUI followed by PDT; and 3) PUI/PS+PDT: PUI of the PS followed by PDT. Microbiological samples were collected from the canals before and after using the protocols described above. The data were analyzed by analysis of variance (Tukey's test) at a significance level of 5%. Microbial counts before PDT, PUI+PDT, and PUI/PS+PDT did not differ significantly ( >0.05), showing methodological standardization in the microbial contamination of the root canal system. There was a significant reduction in microbial counts after PDT (61.05%), PUI+PDT (65.04%), and PUI/PS+PDT (68.58%) ( <0.01), but with no statistically significant difference between the three groups ( >0.05). Based on the present findings, it was concluded that the combination of PUI with PDT or PS activation did not influence microbial reduction achieved by PDT alone.
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ISSN:1735-7497
2008-2746
DOI:10.22037/iej.v19i2.36934