Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil

Context: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. Objective: This paper describes the chemical profile, anti...

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Published in:	Pharmaceutical biology Vol. 55; no. 1; pp. 1884 - 1893
Main Authors:	Xavier, Jadriane de Almeida, Valentim, Iara Barros, Camatari, Fabiana O. S., de Almeida, Alberto M. M., Goulart, Henrique Fonseca, Ferro, Jamylle Nunes de Souza, Barreto, Emiliano de Oliveira, Cavalcanti, Bruno Coelho, Bottoli, Carla B. G., Goulart, Marília Oliveira Fonseca
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 2017 Taylor & Francis Ltd Taylor & Francis Group
Subjects:	Aging Animals Antineoplastic Agents - adverse effects Antineoplastic Agents - chemistry Antineoplastic Agents - isolation & purification Antineoplastic Agents - pharmacology Antioxidants Antioxidants - adverse effects Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Bioactive compounds Biological activity Biological Products - adverse effects Biological Products - chemistry Biological Products - isolation & purification Biological Products - pharmacology biologically active phenols Brazil Cell Line Cell Line, Tumor Cell Survival - drug effects chemical profile Chromatography Chromatography, High Pressure Liquid content of organic volatiles Cricetinae Cricetulus Cytotoxicity Drug Discovery Ethanol Gas chromatography Glycosylation High-performance liquid chromatography Humans Hypoglycemic Agents - adverse effects Hypoglycemic Agents - chemistry Hypoglycemic Agents - isolation & purification Hypoglycemic Agents - pharmacology Lipid peroxidation Lipid Peroxidation - drug effects Macrophages - drug effects Macrophages - immunology Macrophages - metabolism Mass spectrometry Mass spectroscopy Mice Molecular Structure Molting Organic compounds Phenolic compounds Phenols Polyphenols - adverse effects Polyphenols - chemistry Polyphenols - isolation & purification Polyphenols - pharmacology Propolis Propolis - chemistry Scientific imaging Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry VOCs Volatile organic compounds Brazil chemical profile biologically active phenols content of organic volatiles
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Summary:	Context: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. Objective: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. Material and methods: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH * , FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04-25 μg/mL concentrations, for 72 h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed. Results: The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC 50 value for anti-glycation activity of EEP was between 16.5 and 19.2 μg/mL, close to aminoguanidine (IC 50 = 7.7 μg/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported. Conclusions: The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Supplemental data for this article can be accessed here.
ISSN:	1388-0209 1744-5116
DOI:	10.1080/13880209.2017.1340962