Natural feed additive containing Saccharomyces cerevisiae-originated free nucleotides improves innate immunity, gut histology and disease resistance in Nile tilapia

Natural feed additive containing Saccharomyces cerevisiae-originated free nucleotides improves innate immunity, gut histology and disease resistance in Nile tilapia

A 60-day feeding trial was conducted to assess the effects of dietary Saccharomyces cerevisiae-originated free nucleotides on growth, innate immunity, intestinal histomorphometry and disease resistance in Nile tilapia, Oreochromis niloticus (average initial weight of 7.85 g). Five diets (treatments)...

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Bibliographic Details
Published in:Animal feed science and technology Vol. 289; p. 115337
Main Authors: Barducci, Robson Sfaciotti, de Abreu, Vanderlei, Santos, Anderson Aparecido Dias, Pacheco, Letícia Graziele, Koch, João Fernando Albers, Florencio, Moisés, Pilarski, Fabiana
Format: Journal Article
Language:English
Published: Elsevier B.V 01-07-2022
Subjects:
Aquaculture feed
Fish disease
Gut histology
Innate immunity
Nucleotide
Yeast
5′GMP.Na2
Yeast
RNA
DI
SRBC
N320
Innate immunity
ASA
UFC
OD
N170
5′UMP.Na2
N470
PBS
NBT
Fish disease
Gut histology
5′CMP
5′AMP
IL-1β
NCD
PCD
TNF-α
Aquaculture feed
DNA
Nucleotide
GRAS
ATP
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Summary:A 60-day feeding trial was conducted to assess the effects of dietary Saccharomyces cerevisiae-originated free nucleotides on growth, innate immunity, intestinal histomorphometry and disease resistance in Nile tilapia, Oreochromis niloticus (average initial weight of 7.85 g). Five diets (treatments) were prepared: negative control diet (NCD) - basal diet without additives; positive control diet (PCD) - basal diet supplemented with a commercial nucleotide-based additive at 170 ppm; and three experimental diets, N170, N320 and N470, supplemented with S. cerevisiae-originated free nucleotides at 170, 320 and 470 ppm, respectively. There were no significant differences in production parameters after the trial period. Nevertheless, the complement hemolytic activity was higher in fish subjected to the three experimental diets, while serum lysozyme concentration was higher in the N470 group compared with both controls. Intestinal villi height and density were greater in fish fed the N470 diet compared with the NCD group. In addition, fish receiving the three experimental diets showed higher absorption surface area magnification ratio, with the greatest values being observed in the N470 group. Lastly, the highest survival rate following Aeromonas hydrophila challenge was recorded in the N470 group. Taken together, the present findings indicate that supplementation of feed with S. cerevisiae-originated free nucleotides at 470 ppm improves non-specific immune responses and intestinal microstructure morphology as well as increases survival rate after bacterial infection in Nile tilapia. Therefore, this blend of natural nucleotides has the potential of being used as an alternative to antimicrobials when included as an additive in aquaculture feeds. •Diets supplemented with Saccharomyces cerevisiae-originated free nucleotides were examined in Nile tilapia.•S. cerevisiae-originated free nucleotides promoted improvements in immunity and intestinal morphology in Nile tilapia.•Diets supplemented with S. cerevisiae-originated free nucleotides increased the survival rate after bacterial infection.
ISSN:0377-8401
1873-2216
DOI:10.1016/j.anifeedsci.2022.115337