Population Study of Seven Microsatellites in Alagoas-Northeastern Brazil

Population Study of Seven Microsatellites in Alagoas-Northeastern Brazil

Last decade DNA profiling STR loci has become widely used for human identification in forensic science and other related fields. Data banks for STR loci have not yet been established in Alagoas population, Brazil. The aim of this study was to carry out the analysis of allele frequencies of the loci...

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Bibliographic Details
Published in:Journal of forensic sciences Vol. 47; no. 6; pp. 1399 - 1400
Main Authors: daSilva, LAF, Pimentel, BJ, de Azevedo, DA, da Silva, ENP, dos Santos, SS
Format: Journal Article
Language:English
Published: United States 01-11-2002
Subjects:
Alleles
Brazil
DNA Fingerprinting - methods
Gene Frequency
Genetics, Population
Humans
Polymerase Chain Reaction
Tandem Repeat Sequences
Brazil
population genetics
D21S11
FGA
D18S51
D10S2325
forensic science
Brazil
LPL
F13B
D3S1358
DNA typing
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Summary:Last decade DNA profiling STR loci has become widely used for human identification in forensic science and other related fields. Data banks for STR loci have not yet been established in Alagoas population, Brazil. The aim of this study was to carry out the analysis of allele frequencies of the loci F13B, LPL, D3S1358, D18S51, D21S11, FGA and D10S2325 in Alagoas, as well as to determine other statistics parameters of forensic and paternity interest. Specimens were collected from over 153 unrelated individuals living in Alagoas region-Northeastern Brazil. DNA was obtained from peripheral blood extracted with Chelex method as previously described (1). PCR reactions were performed and analysis of the products was made by polyacrylamide gel electrophoresis silver stained. Allele frequencies and others statistics parameters for forensic and paternity were determined for each locus by the Power Stats software (http://www.promega.com/techserv/apps/hmnid)-Promega Corporation, USA (2). Observed and expected heterozigosity as well as calculations for Hardy-Weinberg Equilibrium were performed in Arlequin 2.0 software (http://lgb.unige.ch/ arlequin) (3). None of the analyzed loci showed deviations from HWE ( p> 0.05) in the population studied. A more complete data set can be accessed at http://www.mhn.ufal.br/forense.
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ISSN:0022-1198
1556-4029
DOI:10.1520/JFS15583J