Protective effect of ions against cell death induced by acid stress in Saccharomyces

Protective effect of ions against cell death induced by acid stress in Saccharomyces

Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest...

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Published in:FEMS yeast research Vol. 9; no. 5; pp. 701 - 712
Main Authors: dos Santos Sant'Ana, Gilzeane, da Silva Paes, Lisvane, Vieira Paiva, Argentino F, Fietto, Luciano Gomes, Totola, Antônio Helvécio, Magalhães Trópia, Maria J, Lemos, Denise Silveira, Lucas, Cândida, Fietto, Juliana L. Rangel, Brandão, Rogelio L, Castro, Ieso de Miranda
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-08-2009
Blackwell Publishing Ltd
Oxford University Press
Subjects:
acid tolerance
Acids - pharmacology
Antifungal Agents - pharmacology
Cell Death
Cell viability
ENA genes
Enteritis
Gastrointestinal diseases
Gene Deletion
Genes, Fungal
Hydrogen
Hydrogen-Ion Concentration
Ions - metabolism
Membrane potential
Microbial Viability
Mutants
Na+/H+-exchanging ATPase
pH effects
probiotic
Probiotics
Proton Pumps - metabolism
Saccharomyces - drug effects
Saccharomyces - physiology
Saccharomyces boulardii
Saccharomyces cerevisiae
Salts
Sodium chloride
Stress, Physiological
genes
probiotic
acid tolerance
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Summary:Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under these conditions, a pH 2.0 was the main factor responsible for decreased cell viability. Importantly, the addition of low concentrations of sodium chloride (NaCl) protected cells in acidic conditions more effectively than other salts. In the absence of S. boulardii mutants, the protective effects of Na⁺ in yeast viability in acidic conditions was tested using S. cerevisiae Na⁺-ATPases (ena1-4), Na⁺/H⁺ antiporter (nha1Δ) and Na⁺/H⁺ antiporter prevacuolar (nhx1Δ) null mutants, respectively. Moreover, we provide evidence suggesting that this protection is determined by the plasma membrane potential, once altered by low pH and low NaCl concentrations. Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells.
Bibliography:http://dx.doi.org/10.1111/j.1567-1364.2009.00523.x
Editor: André Goffeau
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2009.00523.x