Combination of natural antimicrobials for contamination control in ethanol production

Presence of bacterial contaminants at levels > 10 7 colony forming units per milliliter (CFU/mL) during ethanol production processes reduces the alcoholic fermentation yield by 30%. Antibiotics are currently used to control contamination, but their residues may be detected in yeast extract, restr...

Full description

Saved in:

Bibliographic Details
Published in:	World journal of microbiology & biotechnology Vol. 35; no. 10; pp. 158 - 9
Main Authors:	Maia, Natalia Janaina Lago, Corrêa, Jessica Audrey Feijó, Rigotti, Rachel Tereza, da Silva Junior, Anisio Antonio, Luciano, Fernando Bittencourt
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-10-2019 Springer Nature B.V
Subjects:	Anti-Bacterial Agents - pharmacology Anti-Infective Agents - pharmacology Antibiotics Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Applied Microbiology Bacteria Baking yeast Biochemistry Biomedical and Life Sciences Biotechnology Contaminants Contamination Environmental Engineering/Biotechnology Ethanol Ethanol - metabolism Fermentation Fermentation - physiology Hops Humulus - chemistry Industrial Microbiology Lactobacillales - drug effects Lactobacillus fermentum - drug effects Leuconostoc mesenteroides Leuconostoc mesenteroides - drug effects Life Sciences Lysozyme Microbial Sensitivity Tests Microbiology Minimum inhibitory concentration Muramidase - pharmacology Nisin Nisin - analogs & derivatives Nisin - pharmacology Original Paper p-Hydroxybenzoic acid Parabens - pharmacology Plant Extracts - pharmacology Pollution control Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharum - chemistry Viability Yeast Yeasts Nisin Hops extract Sugarcane mills Lactic-acid bacteria Antimicrobial formula
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Presence of bacterial contaminants at levels > 10 7 colony forming units per milliliter (CFU/mL) during ethanol production processes reduces the alcoholic fermentation yield by 30%. Antibiotics are currently used to control contamination, but their residues may be detected in yeast extract, restricting this by-product trade to several countries. Thus, the objective of this study was to assess antimicrobial activity of the natural compounds hops extract, 4-hydroxybenzoic acid, nisin Z, and lysozyme against Lactobacillus fermentum , Leuconostoc mesenteroides , and Saccharomyces cerevisiae , aiming development of a formula. Minimum Inhibitory Concentration of each antimicrobial was determined for bacteria and subsequently, nisin (30 mg/L) and hops extract (5 mg/L) were tested together, showing inhibitory effects combining doses of each antimicrobial that were equivalent to an eightfold reduction of their original Minimum Inhibitory Concentrations (3.75 and 0.625 mg/L, respectively), resulting in a FIC Index of 0.25. Thereon, a formula containing both compounds was developed and tested in fermentation assays, promoting reductions on bacterial population and no severe interferences in yeast viability or population even at extreme doses. Therefore, these compounds have great potential to successfully substitute conventional antibiotics in the ethanol industry. Graphic abstract
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0959-3993 1573-0972
DOI:	10.1007/s11274-019-2734-6