Genotoxicity in Oreochromis niloticus (Cichlidae) induced by Microcystis spp bloom extract containing microcystins

Studies of genotoxicity in fish caused by cyanobacterial extracts containing microcystins (MCs) can be useful in determining their carcinogenic risk due to a genotoxic mechanism. An extract of cyanobacterial Microcystis ssp, containing MC-LR and -LA from a bloom collected in a eutrophic lake, showed...

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Bibliographic Details
Published in:	Toxicon (Oxford) Vol. 58; no. 3; pp. 259 - 264
Main Authors:	da Silva, R.R. Pavan, Pires, O.R., Grisolia, C.K.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01-09-2011 Elsevier
Subjects:	Animal poisons toxicology. Antivenoms Animals Apoptosis Apoptosis - drug effects Bacteriology Bioassay Biological and medical sciences Brackish carcinogenicity Cichlids - genetics Comet assay Damage Damage detection Deoxyribonucleic acid DNA Damage erythrocytes fish Fish micronucleus Freshwater Fundamental and applied biological sciences. Psychology Genotoxicity intraperitoneal injection lakes Medical sciences Microbiology Microcystin microcystins Microcystins - toxicity Microcystis Microcystis - chemistry Microcystis - pathogenicity Micronuclei, Chromosome-Defective Mutagenicity Tests Necrosis Oreochromis niloticus Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains risk Toxicology Microcystin Fish micronucleus Comet assay Necrosis Apoptosis Toxicity Genotoxicity Cichlidae Animal origin Extract Microcystis Vertebrata Oreochromis niloticus Cell death Pisces Animal Cyanobacteria Bloom Bacteria Genotoxity test
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Summary:	Studies of genotoxicity in fish caused by cyanobacterial extracts containing microcystins (MCs) can be useful in determining their carcinogenic risk due to a genotoxic mechanism. An extract of cyanobacterial Microcystis ssp, containing MC-LR and -LA from a bloom collected in a eutrophic lake, showed genotoxicity to Oreochromis niloticus. DNA damage (comet assay) was significantly induced in peripheral erythrocytes with both tested concentrations of 6.90 μg kg−1 bw and 13.80 μg kg−1 bw through intraperitoneal injection (ip). There was no micronucleus induction after ip injection at concentrations of 6.90 μg kg−1 bw and 13.80 μg kg−1 bw. Body exposure resulted in micronucleus induction and DNA damage only at the highest tested concentrations of 103.72 μg L−1. Thus, comet assay and ip injection revealed the highest levels of the genotoxicity of MCs. Apoptosis-necrosis test carried out at concentrations of 6.90 μg kg−1 bw and 13.80 μg kg−1 bw revealed that at low concentrations more apoptosis than necrosis occurred. At higher concentrations more necrosis than apoptosis occurred. ► Microcystin showed genotoxicity to Oreochromis niloticus fish. ► Microcystin body exposure resulted in micronucleus induction and DNA damage. ► At lower concentrations more apoptosis than necrosis occurred. ► At higher concentrations more necrosis was observed.
Bibliography:	http://dx.doi.org/10.1016/j.toxicon.2011.06.005 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0041-0101 1879-3150
DOI:	10.1016/j.toxicon.2011.06.005