Mesenchymal stem cells from sternum: the type of heart disease, ischemic or valvular, does not influence the cell culture establishment and growth kinetics

In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. To study the proliferative and differentiati...

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Published in:	Journal of translational medicine Vol. 15; no. 1; p. 161
Main Authors:	Dias, Lucinara Dadda, Casali, Karina Rabello, Ghem, Carine, da Silva, Melissa Kristocheck, Sausen, Grasiele, Palma, Patrícia Bonini, Covas, Dimas Tadeu, Kalil, Renato A K, Schaan, Beatriz D, Nardi, Nance Beyer, Markoski, Melissa Medeiros
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 25-07-2017 BioMed Central BMC
Subjects:	Age Aged Blood diseases Bone marrow Cardiovascular disease Cardiovascular diseases Cell culture Cell Culture Techniques - methods Cell cycle Cell Differentiation Cell Proliferation Cell Separation Cell Shape Cell therapy Cells, Cultured Centrifugation Colony-forming cells Colony-Forming Units Assay Comparative analysis Coronary artery disease Dyslipidemia Female Growth Growth kinetics Health aspects Health risks Heart Heart diseases Heart Valve Diseases - pathology Humans Immunophenotyping Ischemia Ischemic heart disease Isolation Kinetics Male Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchyme Middle Aged MSC establishment Myocardial Ischemia - pathology Pathophysiology Physiological aspects Regeneration Rheumatic heart disease Risk factors Senescence Statins Stem cells Stem cells culture Sternum Sternum - cytology Studies Tissue engineering Valvular heart disease Ischemic heart disease Valvular heart disease MSC establishment Cell therapy Stem cells culture
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Summary:	In an attempt to increase the therapeutic potential for myocardial regeneration, there is a quest for new cell sources and types for cell therapy protocols. The pathophysiology of heart diseases may affect cellular characteristics and therapeutic results. To study the proliferative and differentiation potential of mesenchymal stem cells (MSC), isolated from bone marrow (BM) of sternum, we made a comparative analysis between samples of patients with ischemic (IHD) or non-ischemic valvular (VHD) heart diseases. We included patients with IHD (n = 42) or VHD (n = 20), with average age of 60 years and no differences in cardiovascular risk factors. BM samples were collected (16.4 ± 6 mL) and submitted to centrifugation with Ficoll-Paque, yielding 4.5 ± 1.5 × 10 cells/mL. Morphology, immunophenotype and differentiation ability had proven that the cultivated sternal BM cells had MSC features. The colony forming unit-fibroblast (CFU-F) frequency was similar between groups (p = 0.510), but VHD samples showed positive correlation to plated cells vs. CFU-F number (r = 0.499, p = 0.049). The MSC culture was established in 29% of collected samples, achieved passage 9, without significant difference in expansion kinetics between groups (p > 0.05). Dyslipidemia and the use of statins was associated with culture establishment for IHD patients (p = 0.049 and p = 0.006, respectively). Together, these results show that the sternum bone can be used as a source for MSC isolation, and that ischemic or valvular diseases do not influence the cellular yield, culture establishment or in vitro growth kinetics.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1479-5876 1479-5876
DOI:	10.1186/s12967-017-1262-0