Evaluation of a new rapid test for carbapenemase detection in carbapenem resistant Enterobacteriaceae

We evaluated a new phenotypic test for carbapenemase detection. A total of 100 Enterobacteriaceae isolates were selected. The test was compared with conventional PCR for blaKPC and blaNDM detection. We found 100% sensitivity and specificity, suggesting that this test may be a feasible alternative fo...

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Published in:	Journal of microbiological methods Vol. 115; pp. 20 - 21
Main Authors:	Martino, Marines Dalla Valle, Koga, Paula Célia Mariko, Pasternak, Jacyr, Doi, André Mario, Ciola, Claudete Silvia, da Silva, Cely Barreto, Massaia, Irineu Francisco Delfino Silva, da Silva, Itacy Gonçalves Siqueira, de Araújo, Maria Rita Elmor
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-08-2015
Subjects:	Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics beta-Lactamases - genetics Carbapenemases Carbapenems - pharmacology Enterobacteriaceae Enterobacteriaceae - drug effects Enterobacteriaceae - enzymology Enterobacteriaceae - genetics Enterobacteriaceae - isolation & purification Enterobacteriaceae Infections - microbiology Humans Phenotypic method Polymerase Chain Reaction - methods Resistance Resistance Phenotypic method Carbapenemases Enterobacteriaceae
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Summary:	We evaluated a new phenotypic test for carbapenemase detection. A total of 100 Enterobacteriaceae isolates were selected. The test was compared with conventional PCR for blaKPC and blaNDM detection. We found 100% sensitivity and specificity, suggesting that this test may be a feasible alternative for rapid carbapenemase detection. [Display omitted] •A new and rapid phenotypic test for carbapenemase detection was evaluated.•This method consists of strips that are impregnated with Imipenem and starch.•The sensitivities and specificities were 100%.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2015.05.001