Proteomic fingerprinting for the fast and accurate identification of species in the Polyporoid and Hymenochaetoid fungi clades

Basidiomycotan fungi play significant roles in the biogeochemical cycle of carbon as wood decomposers and are used in the food industry for mushroom production and in biotechnology for the production of diverse bioactive compounds and bioremediation. The correct identification of basidiomycotan isol...

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Bibliographic Details
Published in:	Journal of proteomics Vol. 203; p. 103390
Main Authors:	Tomé, Luiz Marcelo Ribeiro, Badotti, Fernanda, Assis, Gabriella Borba Netto, Fonseca, Paula Luize Camargos, da Silva, Genivaldo Alves, da Silveira, Rosa Mara Borges, Costa-Rezende, Diogo Henrique, dos Santos, Elisandro Ricardo Drechsler, de Carvalho Azevedo, Vasco Ariston, Figueiredo, Henrique Cesar Pereira, Góes-Neto, Aristóteles
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 15-07-2019
Subjects:	Basidiomycota Fungal identification Intact cell mass spectrometry MALDI-TOF MS Proteomic fingerprinting Fungal identification MALDI-TOF MS Proteomic fingerprinting Basidiomycota Intact cell mass spectrometry
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Summary:	Basidiomycotan fungi play significant roles in the biogeochemical cycle of carbon as wood decomposers and are used in the food industry for mushroom production and in biotechnology for the production of diverse bioactive compounds and bioremediation. The correct identification of basidiomycotan isolates is crucial for understanding their biology and being able to expand their applications. Currently, the identification of these organisms is performed by analyzing morphological and genomic characteristics, primarily those based on DNA biomarkers. Despite their efficiency, such methods require considerable expertise and are both time-consuming and error-prone (multistep workflow). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged in the last decade as an accurate, fast, and powerful alternative for the identification of microorganisms. MALDI-TOF MS has been widely applied for the identification and taxonomical characterization of both bacteria and ascomycotan fungi from clinical origins. However, species of Basidiomycota have been poorly evaluated using this method. In the present study, we assessed the performance of MALDI-TOF MS using basidiomycotan isolates of two distinct taxonomical families: Polyporaceae and Hymenochaetaceae. Using a simple protocol, which eliminates the protein extraction step, we obtained high-quality mass spectra data and demonstrated that this method is efficient for the discrimination of isolates at the species level. In this study, the MALDI-TOF mass spectrometry was employed to test its accuracy on the recognition of fungal species with high biotechnological and environmental interest. Using a simple and fast protocol, we obtained high-quality mass-spectra (protein fingerprinting) and proved that MALDI-TOF MS is sufficiently robust to the identification at species level and to evaluate the relationships among the isolates of the polyporoid and hymenochaetoid clades (Basidiomycota). [Display omitted] •High-quality mass spectra were obtained using a simple and fast protocol.•Growth time influenced the protein fingerprinting.•Polyporoid and hymenochaetoid isolates were accurately identified using MALDI-TOF.•Protein fingerprinting was suitable to evaluate the relationship among the isolates.
ISSN:	1874-3919
DOI:	10.1016/j.jprot.2019.103390