Search Results - "da Silva, C Balao"
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Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation
Published in Reproduction (Cambridge, England) (01-12-2016)“…To investigate the hypothesis that oxidative phosphorylation is a major source of ATP to fuel stallion sperm motility, oxidative phosphorylation was suppressed…”
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Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility
Published in PloS one (25-09-2015)“…Mitochondria have been proposed as the major source of reactive oxygen species in somatic cells and human spermatozoa. However, no data regarding the role of…”
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Sex sorting increases the permeability of the membrane of stallion spermatozoa
Published in Animal reproduction science (01-05-2013)“…At present, the only repeatable means of selecting the sex of offspring is the Beltsville semen sorting technology using flow cytometry (FC). This technology…”
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Testicular perfusion after standing laparoscopic peritoneal flap hernioplasty in stallions
Published in Theriogenology (15-09-2015)“…Acquired inguinal herniation is a very common condition in stallions, usually leading to unilateral or bilateral castration to prevent future recurrence…”
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Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA
Published in Reproduction in domestic animals (01-02-2016)“…To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this…”
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Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Tradtitional Protocols
Published in Reproduction in domestic animals (01-12-2014)“…Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly…”
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Dimethylformamide Improves the In vitro Characteristics of Thawed Stallion Spermatozoa Reducing Sublethal Damage
Published in Reproduction in domestic animals (01-12-2012)“…A total of 42 ejaculates were used in the experiment; six ejaculates per stallion, obtained from seven Pure Spanish stallions (PRE), were split and frozen in…”
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Impact of Reproductive Technologies on Stallion Mitochondrial Function
Published in Reproduction in domestic animals (01-08-2015)“…The traditional assessment of stallion sperm comprises evaluation of sperm motility and membrane integrity and identification of abnormal morphology of the…”
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Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential
Published in Theriogenology (15-04-2012)“…Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been…”
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Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na+
Published in Andrology (Oxford) (01-11-2017)“…Summary In order to gain insight of the modifications that freezing and thawing cause to the surviving population of spermatozoa, changes in the potential of…”
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Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis
Published in Reproduction in domestic animals (01-02-2018)“…Contents Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer…”
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Effect of sex sorting on stallion spermatozoa: Heterologous oocyte binding, tyrosine phosphorylation and acrosome reaction assay
Published in Animal reproduction science (01-09-2013)“…The interest on sex sorting by flow cytometry on the equine industry has been increasing over the years. In this work, three different tests were performed in…”
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Caspase Activation, Hydrogen Peroxide Production and Akt Dephosphorylation Occur During Stallion Sperm Senescence
Published in Reproduction in domestic animals (01-08-2014)“…To investigate the mechanisms inducing sperm death after ejaculation, stallion ejaculates were incubated in BWW media during 6 h at 37°C. At the beginning of…”
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Effect of Hoechst 33342 on stallion spermatozoa incubated in KMT or Tyrodes modified INRA96
Published in Animal reproduction science (01-04-2012)“…The only known means of effectively separating populations of X and Y bearing sperms is the Beltsville sexing technology. The technology implies that each…”
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During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa
Published in Animal reproduction science (01-02-2014)“…To investigate the role of the processed autophagy marker light chain 3 (LC3B) protein in sperm survival in stallion semen processing during cooled storage,…”
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Effect of freezing and thawing on TNFα and its receptors TNFR1 and TNFR2 in stallion spermatozoa
Published in Journal of equine veterinary science (01-08-2012)Get full text
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Apoptosis related protein profile in cooled stallion semen
Published in Journal of equine veterinary science (01-08-2012)Get full text
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Effect of sex sorting on intracellular Ca2+ and ATP content of stallion spermatozoa
Published in Journal of equine veterinary science (01-08-2012)Get full text
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