EFFECT OF SOLID MEDIUM DURING COOLED STORAGE ON STALLION SPERM PARAMETERS

Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians. This experiment aims to evaluate the ef...

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Bibliographic Details
Published in:	Cryo-Letters Vol. 36; no. 5; p. 313
Main Authors:	Santos, F C, Corcini, C D, Costa, V G, Gheller, S M, Nogueira, C E, da Rosa Curcio, B, Varel, Jr, A S
Format:	Journal Article
Language:	English
Published:	England 01-09-2015
Subjects:	Acrosome - drug effects Acrosome - metabolism Animals Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents - pharmacology Gelatin - pharmacology Horses - physiology Male Semen - cytology Semen - drug effects Semen - metabolism Semen Analysis Semen Preservation - methods Semen Preservation - veterinary Sperm Motility - drug effects Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - metabolism
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Summary:	Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians. This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms. Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments: control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%, 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5 degree C. Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period. Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.
ISSN:	0143-2044