Atorvastatin inhibits IL-17A, TNF, IL-6, and IL-10 in PBMC cultures from patients with severe rheumatoid arthritis

Atorvastatin inhibits IL-17A, TNF, IL-6, and IL-10 in PBMC cultures from patients with severe rheumatoid arthritis

•Atorvastatin decreased IL-17A, TNF, IL-6 and IL-10 levels in PBMC cultures from patients with rheumatoid arthritis.•The Atorvastatin inhibition profile was more evident in patients with more severe disease activity.•Non-responders to in vitro corticosteroid treatment were not sensitize to atorvasta...

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Published in:Immunobiology (1979) Vol. 225; no. 3; p. 151908
Main Authors: de Oliveira, Priscilla Stela Santana, da Paixão, Adson Belém Ferreira, da Rocha Junior, Laurindo Ferreira, Branco Pinto Duarte, Angela Luzia, Pereira, Michelly Cristiny, Barreto de Melo Rêgo, Moacyr Jesus, da Rocha Pitta, Ivan, da Rocha Pitta, Maira Galdino
Format: Journal Article
Language:English
Published: Netherlands Elsevier GmbH 01-05-2020
Subjects:
Clinical assessment
Cytokines
Inflammation
Joint
Statin
Inflammation
Statin
Cytokines
Joint
Clinical assessment
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Summary:•Atorvastatin decreased IL-17A, TNF, IL-6 and IL-10 levels in PBMC cultures from patients with rheumatoid arthritis.•The Atorvastatin inhibition profile was more evident in patients with more severe disease activity.•Non-responders to in vitro corticosteroid treatment were not sensitize to atorvastatin effects. Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint damage, and it may present with comorbidities at the systemic level. The Th1/Th2/Th17 CD4+ lymphocyte imbalance produces inflammatory cytokines, which begin to act, injuring joint tissue. Atorvastatin is a cholesterol- lowering drug with a range of biological effects including anti-inflammatory potential. Patients with rheumatoid arthritis who used statins exhibited clinical improvement. However, the mechanism is not fully understood. Therefore, we aimed to evaluate the RA immunomodulatory activity of atorvastatin. Peripheral blood mononuclear cells (PBMCs) of RA patients and healthy donors were exposed to atorvastatin in different concentrations following a cytotoxicity assay. Th1, Th2, and Th17 cytokines profiles were evaluated in the culture supernatant by cytometric bead array (CBA). Data were analyzed using the Wilcoxon test, and differences were considered significant when p < 0.05. Atorvastatin showed no toxicity at the tested doses in RA PBMC cultures, and at 10μM, it showed the most significant results, reducing IL-17A (p = 0.002), TNF (p = 0.002), and IL-6 (p = 0.008) supernatant levels. The outcomes also revealed that only patients with more severe disease activity and those sensitive to corticoid treatments were responders to atorvastatin in vitro. These findings suggest the potential immunomodulatory action of atorvastatin as a mechanism in rheumatoid arthritis treatment.
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ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2020.151908