Comparative proteomic analysis of somatic embryo maturation in Carica papaya L

Comparative proteomic analysis of somatic embryo maturation in Carica papaya L

Somatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and unde...

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Bibliographic Details
Published in:Proteome science Vol. 12; no. 1; p. 37
Main Authors: Vale, Ellen de Moura, Heringer, Angelo Schuabb, Barroso, Tatiana, Ferreira, André Teixeira da Silva, da Costa, Monique Nunes, Perales, Jonas Enrique Aguilar, Santa-Catarina, Claudete, Silveira, Vanildo
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 26-06-2014
BioMed Central
Subjects:
Analysis
Embryonic development
Embryos
Fruits
Genetic aspects
Mass spectrometry
Peptides
Physiological aspects
Polyethylene glycol
Proteins
Studies
Germany
Two-dimensional electrophoresis
Papaya
Protein profile
Mass spectrometry
Somatic embryogenesis
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Summary:Somatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and understanding of different biological responses. In addition, the identification of molecular markers could lead to the optimization of protocols allowing the use of biotechnology for papaya propagation and reproduction. This work aimed to investigate the effects of polyethylene glycol (PEG) on somatic embryo development and the protein expression profile during somatic embryo maturation in papaya (Carica papaya L.). The maturation treatment supplemented with 6% PEG (PEG6) resulted in the greatest number of somatic embryos and induced differential protein expression compared with cultures grown under the control treatment. Among 135 spots selected for MS/MS analysis, 76 spots were successfully identified, 38 of which were common to both treatments, while 14 spots were unique to the control treatment, and 24 spots were unique to the PEG6 treatment. The identified proteins were assigned to seven categories or were unclassified. The most representative class of proteins observed in the control treatment was associated with the stress response (25.8%), while those under PEG6 treatment were carbohydrate and energy metabolism (18.4%) and the stress response (18.4%). The differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya.
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ISSN:1477-5956
1477-5956
DOI:10.1186/1477-5956-12-37