Poly(ethylene terephthalate) (PET) degradation by Yarrowia lipolytica: Investigations on cell growth, enzyme production and monomers consumption

Poly(ethylene terephthalate) (PET) degradation by Yarrowia lipolytica: Investigations on cell growth, enzyme production and monomers consumption

[Display omitted] •Yarrowia lipolytica enzymatically hydrolyzes poly(ethylene terephthalate).•Poly(ethylene terephthalate) monomers may act as inducers in the process of lipase production.•Microbiological degradation needs to be further studied to become competitive.•Mono(2-hydroxyethyl) terephthala...

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Bibliographic Details
Published in:Process biochemistry (1991) Vol. 95; no. 11; pp. 81 - 90
Main Authors: da Costa, Andressa Maio, de Oliveira Lopes, Verônica Regina, Vidal, Léa, Nicaud, Jean-Marc, de Castro, Aline Machado, Coelho, Maria Alice Zarur
Format: Journal Article
Language:English
Published: Barking Elsevier Ltd 01-08-2020
Elsevier BV
Elsevier
Subjects:
Biocatalysis
Biodegradation
Catalysis
Consumption
Culture media
Depolymerization
Environmental impact
Ethylene
Glucose
Hydrolysis
Life Sciences
Lipase
Monomers
Oligomers
PET recycling
Poly(ethylene terephthalate)
Polyethylene terephthalate
Polymers
Terephthalic acid
Yarrowia lipolytica
Yeast
Yeasts
Depolymerization
PET recycling
Biocatalysis
Lipase
Poly(ethylene terephthalate)
Yarrowia lipolytica
environmental impacts
packages
monomers consumption
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Summary:[Display omitted] •Yarrowia lipolytica enzymatically hydrolyzes poly(ethylene terephthalate).•Poly(ethylene terephthalate) monomers may act as inducers in the process of lipase production.•Microbiological degradation needs to be further studied to become competitive.•Mono(2-hydroxyethyl) terephthalate was the main intermediate released.•Enzymatic depolymerization of poly(ethylene terephthalate) is a promising alternative. The development of technologies for poly(ethylene terephthalate) (PET) depolymerization, such as biocatalysis, has been pointed as a very promising alternative to chemical hydrolysis processes. This work aims to understand the behavior of Yarrowia lipolytica, a robust yeast for diverse applications, in the presence of molecules from the PET production chain such as monoethylene glycol (MEG), terephthalic acid (TPA), bis (2-hydroxyethyl) terephthalate (BHET), PET oligomers, amorphous PET and post-consumer PET. The yeast was cultivated in rich media with and without glucose addition, in order to compare monomers release or consumption. TPA and MEG were consumed more intensely in the absence of glucose. The addition of the diester BHET yielded a 3-fold increased lipase production both at 160 rpm (118 U/L) and 250 rpm (385 U/L). In addition, 250 rpm agitation also provided a higher consumption of TPA (26% increase). The mono(2-hydroxyethyl) terephthalate (MHET) was the main intermediate released during polymer hydrolysis, followed by TPA and BHET. Thus, the use of Yarrowia lipolytica, which is capable of catalyzing the PET hydrolysis is of great potential to reduce the environmental impacts caused by unappropriated disposal of packages.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2020.04.001