Cranberry (Vaccinium macrocarpon) peel polyphenol-rich extract attenuates rat liver mitochondria impairments in alcoholic steatohepatitis in vivo and after oxidative treatment in vitro

[Display omitted] •Flavan-3-ols, procyanidins, flavonols, and phenolic acids were identified in cranberry peel.•Ethanol-induced steatohepatitis in rats resulted in liver mitochondria dysfunction.•Cranberry polyphenols demonstrated hepatoprotective and anti-inflammatory activities.•Isolated polypheno...

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Bibliographic Details
Published in:	Journal of functional foods Vol. 57; pp. 83 - 94
Main Authors:	Zavodnik, Ilya, Buko, Vyacheslav, Lukivskaya, Oxana, Lapshina, Elena, Ilyich, Tatsiana, Belonovskaya, Elena, Kirko, Siarhei, Naruta, Elena, Kuzmitskaya, Irina, Budryn, Grazyna, Zyzelevicz, Dorota, Orach, Joanna, Zakrzeska, Agnieszka, Kiryukhina, Lyudmila
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 01-06-2019 Elsevier
Subjects:	Alcoholic steatohepatitis Cranberry peel polyphenols Liver Mitochondrial permeability transition Mitochondrial ultrastructure Alcoholic steatohepatitis RCR Mitochondrial permeability transition AST TNFα tBHP Liver MS amu ALT TBARS IL-6 Cranberry peel polyphenols Mitochondrial ultrastructure CsA MPT ASH ROS TGFβ CPE tR
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Summary:	[Display omitted] •Flavan-3-ols, procyanidins, flavonols, and phenolic acids were identified in cranberry peel.•Ethanol-induced steatohepatitis in rats resulted in liver mitochondria dysfunction.•Cranberry polyphenols demonstrated hepatoprotective and anti-inflammatory activities.•Isolated polyphenols with antioxidant properties restored mitochondrial functions. Alcoholic steatohepatitis is an important medical problem but its effective therapies are still not available. Plant polyphenols are widely used for prevention of toxic liver damages. The aim of the study was to evaluate the hepatoprotective mechanism(s) of a cranberry peel polyphenol-rich extract, focusing on the effects of the polyphenols on the mitochondrial function. The main components of cranberry peel phenolic compounds were anthocyanins, flavan-3-ols, procyanidins, flavonols, phenolic acids, as was detected using UHPLC-ESI-QTOF-MS. The treatment of rats receiving ethanol (4 g/kg bw, 8 weeks) with cranberry polyphenols (daily, 4 mg/kg bw) partially prevented alcoholic liver damage, ameliorating steatosis and inflammatory signs in the liver, decreasing serum and liver triglyceride contents, ALT and AST activities, as well as diminishing TNFα and TGFβ levels in serum. The polyphenols inhibited Ca2+ - induced mitochondrial permeability transition, free radical generation in mitochondria during intoxication. The polyphenols (25 µg/ml) prevented mitochondrial oxidative impairments in vitro. In conclusion, the cranberry peel polyphenols with antioxidant properties exerted a hepatoprotective and anti-inflammatory effects in the model of alcoholic steatohepatitis via prevention of liver mitochondria dysfunction.
ISSN:	1756-4646 2214-9414
DOI:	10.1016/j.jff.2019.04.001