A New Human Uveal Melanoma Cell Line: Melanin Production and Molecular Markers for Targeted Therapy

A new human uveal melanoma (UM) cell line uMel1 was established by mechanical disintegration of a tumor fragment. uMel1 cells had a stellate dendrite-like shape, contained a lot of brown melanin pigment, and had a low mitotic index. Optimization of cultivation conditions led to an increase in the ra...

Full description

Saved in:

Bibliographic Details
Published in:	Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 17; no. 4; pp. 165 - 171
Main Authors:	Zhilnikova, M. V., Novak, D. D., Troitskaya, O. S., Nushtaeva, A. A., Biryukov, M. M., Zvereva, S. P., Varlamov, M. E., Koval, V. V., Stanishevskaya, O. M., Chernikh, D. V., Kononova, N. V., Atamanov, V. V., Koval, O. A.
Format:	Journal Article
Language:	English
Published:	Moscow Pleiades Publishing 01-12-2023 Springer Nature B.V
Subjects:	Bioorganic Chemistry Cell adhesion molecules Cell culture Cell proliferation Cell viability Chemistry Chemistry and Materials Science Dihydroxyphenylalanine E-cadherin ErbB protein ErbB-2 protein Flow cytometry Levodopa Medicinal Chemistry Melanin Melanocytes Melanoma N-Cadherin Phenotypes primary cell cultures targeted therapy uveal melanoma Mel-CAM L-DOPA
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A new human uveal melanoma (UM) cell line uMel1 was established by mechanical disintegration of a tumor fragment. uMel1 cells had a stellate dendrite-like shape, contained a lot of brown melanin pigment, and had a low mitotic index. Optimization of cultivation conditions led to an increase in the rate of cell proliferation and was accompanied by the loss of brown pigment. Since the melanin precursor is L-dihydroxyphenylalanine (L-DOPA), the authors analyzed the cultivation of uMel1 cells in the presence of L-DOPA. When L-DOPA was used at a concentration of 20 μg/mL, causing a decrease in cell viability by no more than 10%, melanocytes uMel1 synthesized melanin. It can be concluded that cultivation in the presence of L-DOPA provides the phenotype of melanin-containing melanocytes of uMel1 personal culture under conditions of long-term cultivation. Analysis of cell adhesion molecules N-cadherin (N-cad), E-cadherin (E-cad), and Mel-CAM, as well as receptors of the epidermal growth factor (ErbB) family by flow cytometry, showed that uMel1 cells have a phenotype of N-cad – /E-cad – /Mel-CAM + /HER2 low /HER3 low , and can be used for the study of targeted drugs to Mel-CAM, HER2 and HER3.
ISSN:	1990-7508 1990-7516
DOI:	10.1134/S1990750823600607