Search Results - "Zuo, Erwei"

Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos by Zuo, Erwei, Sun, Yidi, Wei, Wu, Yuan, Tanglong, Ying, Wenqin, Sun, Hao, Yuan, Liyun, Steinmetz, Lars M, Li, Yixue, Yang, Hui

“…Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to determine off-target effects of editing due to single-nucleotide…”
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A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects by Zuo, Erwei, Sun, Yidi, Yuan, Tanglong, He, Bingbing, Zhou, Changyang, Ying, Wenqin, Liu, Jing, Wei, Wu, Zeng, Rong, Li, Yixue, Yang, Hui

“…Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in…”
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Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods by Yuan, Tanglong, Yan, Nana, Fei, Tianyi, Zheng, Jitan, Meng, Juan, Li, Nana, Liu, Jing, Zhang, Haihang, Xie, Long, Ying, Wenqin, Li, Di, Shi, Lei, Sun, Yongsen, Li, Yongyao, Li, Yixue, Sun, Yidi, Zuo, Erwei

“…Efficient and precise base editors (BEs) for C-to-G transversion are highly desirable. However, the sequence context affecting editing outcome largely remains…”
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CRISPR/Cas9-mediated targeted chromosome elimination by Zuo, Erwei, Huo, Xiaona, Yao, Xuan, Hu, Xinde, Sun, Yidi, Yin, Jianhang, He, Bingbing, Wang, Xing, Shi, Linyu, Ping, Jie, Wei, Yu, Ying, Wenqin, Wei, Wei, Liu, Wenjia, Tang, Cheng, Li, Yixue, Hu, Jiazhi, Yang, Hui

“…The CRISPR/Cas9 system has become an efficient gene editing method for generating cells carrying precise gene mutations, including the rearrangement and…”
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Mitochondrial base editor DdCBE causes substantial DNA off-target editing in nuclear genome of embryos by Wei, Yinghui, Li, Zhifang, Xu, Kui, Feng, Hu, Xie, Long, Li, Di, Zuo, Zhenrui, Zhang, Meiling, Xu, Chunlong, Yang, Hui, Zuo, Erwei

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GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos by Zuo, Erwei, Sun, Yidi, Wei, Wu, Yuan, Tanglong, Ying, Wenqin, Sun, Hao, Yuan, Liyun, Steinmetz, Lars M., Li, Yixue, Yang, Hui

“…Genome editing holds great potential for correcting pathogenic mutations. We developed a method called GOTI (genome-wide off-target analysis by two-cell embryo…”
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One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs by Zuo, Erwei, Cai, Yi-Jun, Li, Kui, Wei, Yu, Wang, Bang-An, Sun, Yidi, Liu, Zhen, Liu, Jiwei, Hu, Xinde, Wei, Wei, Huo, Xiaona, Shi, Linyu, Tang, Cheng, Liang, Dan, Wang, Yan, Nie, Yan-Hong, Zhang, Chen-Chen, Yao, Xuan, Wang, Xing, Zhou, Changyang, Ying, Wenqin, Wang, Qifang, Chen, Ren-Chao, Shen, Qi, Xu, Guo-Liang, Li, Jinsong, Sun, Qiang, Xiong, Zhi-Qi, Yang, Hui

“…The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion…”
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Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice by Yan, Nana, Feng, Hu, Sun, Yongsen, Xin, Ying, Zhang, Haihang, Lu, Hongjiang, Zheng, Jitan, He, Chenfei, Zuo, Zhenrui, Yuan, Tanglong, Li, Nana, Xie, Long, Wei, Wu, Sun, Yidi, Zuo, Erwei

“…Base editors have been reported to induce off-target mutations in cultured cells, mouse embryos and rice, but their long-term effects in vivo remain unknown…”
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Mini-dCas13X-mediated RNA editing restores dystrophin expression in a humanized mouse model of Duchenne muscular dystrophy by Li, Guoling, Jin, Ming, Li, Zhifang, Xiao, Qingquan, Lin, Jiajia, Yang, Dong, Liu, Yuanhua, Wang, Xing, Xie, Long, Ying, Wenqin, Wang, Haoqiang, Zuo, Erwei, Shi, Linyu, Wang, Ning, Chen, Wanjin, Xu, Chunlong, Yang, Hui

“…Approximately 10% of monogenic diseases are caused by nonsense point mutations that generate premature termination codons (PTCs), resulting in a truncated…”
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Genome-wide CRISPR off-target prediction and optimization using RNA-DNA interaction fingerprints by Chen, Qinchang, Chuai, Guohui, Zhang, Haihang, Tang, Jin, Duan, Liwen, Guan, Huan, Li, Wenhui, Li, Wannian, Wen, Jiaying, Zuo, Erwei, Zhang, Qing, Liu, Qi

“…The powerful CRISPR genome editing system is hindered by its off-target effects, and existing computational tools achieved limited performance in genome-wide…”
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Undetectable off-target effects induced by FokI catalytic domain in mouse embryos by Xie, Long, Feng, Hu, Li, Zhifang, Li, Di, Yang, Xiali, Yuan, Tanglong, Yan, Nana, He, Chenfei, Zheng, Jitan, Zuo, Zhenrui, Zheng, Yaxuan, Cao, Yaqi, Lu, Yangqing, Xiong, Xing Yao, Zuo, Erwei

“…The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target…”
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Overexpression of miRNA29a gene inhibits proliferation and promotes apoptosis of jejunal epithelial cells in yak by Jia, Jianlei, Zuo, Erwei, Li, Ning, Kong, Siyuan, Bao, Pengjia, Chen, Qian, Yan, Ping

“…MicroRNAs (miRNAs) were identified to be involved in various biological functions by regulating the degradation or suppressing the translation of their…”
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Author Correction: Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation by Li, Guoling, Dong, Xue, Luo, Jiamin, Yuan, Tanglong, Li, Tong, Zhao, Guoli, Zhang, Hainan, Zhou, Jingxing, Zeng, Zhenhai, Cui, Shuna, Wang, Haoqiang, Wang, Yin, Yu, Yuyang, Yuan, Yuan, Zuo, Erwei, Xu, Chunlong, Huang, Jinhai, Zhou, Yingsi

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Engineering TadA ortholog-derived cytosine base editor without motif preference and adenosine activity limitation by Li, Guoling, Dong, Xue, Luo, Jiamin, Yuan, Tanglong, Li, Tong, Zhao, Guoli, Zhang, Hainan, Zhou, Jingxing, Zeng, Zhenhai, Cui, Shuna, Wang, Haoqiang, Wang, Yin, Yu, Yuyang, Yuan, Yuan, Zuo, Erwei, Xu, Chunlong, Huang, Jinhai, Zhou, Yingsi

“…The engineered TadA variants used in cytosine base editors (CBEs) present distinctive advantages, including a smaller size and fewer off-target effects…”
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PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis by Wang, Xiukun, Kang, Jun-Yan, Wei, Leixin, Yang, Xiaogan, Sun, Hongduo, Yang, Suming, Lu, Lei, Yan, Meng, Bai, Meizhu, Chen, Yanyan, Long, Juanjuan, Li, Na, Li, Dangsheng, Huang, Jing, Lei, Ming, Shao, Zhen, Yuan, Wen, Zuo, Erwei, Lu, Kehuan, Liu, Mo-Fang, Li, Jinsong

“…Epigenetic regulation, including histone-to-protamine exchanges, controls spermiogenesis. However, the underlying mechanisms of this regulation are largely…”
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Base editing-mediated splicing correction therapy for spinal muscular atrophy by Lin, Xiang, Chen, Haizhu, Lu, Ying-Qian, Hong, Shunyan, Hu, Xinde, Gao, Yanxia, Lai, Lu-Lu, Li, Jin-Jing, Wang, Zishuai, Ying, Wenqin, Ma, Lixiang, Wang, Ning, Zuo, Erwei, Yang, Hui, Chen, Wan-Jin

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Develop an efficient and specific AAV-based labeling system for Muller glia in mice by Gao, Yanxia, Fang, Kailun, Yan, Zixiang, Zhang, Haiwei, Geng, Guannan, Wu, Weiwei, Xu, Ding, Zhang, Heng, Zhong, Na, Wang, Qifang, Cai, Minqing, Zuo, Erwei, Yang, Hui

“…Reprogramming Müller glia (MG) into functional cells is considered a promising therapeutic strategy to treat ocular diseases and vision loss. However, current…”
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Human cleaving embryos enable efficient mitochondrial base-editing with DdCBE by Wei, Yinghui, Xu, Chunlong, Feng, Hu, Xu, Kui, Li, Zhifang, Hu, Jing, Zhou, Ling, Wei, Yu, Zuo, Zhenrui, Zuo, Erwei, Li, Wen, Yang, Hui, Zhang, Meiling

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The Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Inherited Diseases by Abdelnour, Sameh A, Xie, Long, Hassanin, Abdallah A, Zuo, Erwei, Lu, Yangqing

“…Clustered regularly interspaced short palindromic repeats (CRISPR) is a promising innovative technology for genomic editing that offers scientists the chance…”
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FokI-RYdCas9 Mediates Nearly PAM-Less and High-Precise Gene Editing in Human Cells by Li, Di, Cao, Yaqi, Xie, Long, He, Chenfei, Jiao, Danrong, Ma, Mengxue, Zuo, Zhenrui, Zuo, Erwei, Yang, Xiaogan

“…The demand for high-precision CRISPR/Cas9 systems in biomedicine is experiencing a notable upsurge. The editing system fdCas9 employs a dual-sgRNA strategy to…”
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