The presence and polymorphism of genes encoding serine protease autotransporters (spate) among the Escherichia coli isolated in the region of Gdansk from children with diarrhea

The group of 96 strains ofEscherichia coli isolated from children with diarrhea were investigated towards the presence and polymorphism of genes encoding autotransporters that belong to the group of proteins named SPATE (Serine Protease Autotransporters ofEnterobacteriaceae). Based on the results of...

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Bibliographic Details
Published in:	Medycyna doświadczalna i mikrobiologia Vol. 59; no. 4; p. 343
Main Authors:	Ziomek, Magdalena, Szweda, Piotr, Kurlenda, Julianna, Kochanowski, Rafał, Sikorska-Wiśniewska, Grazyna
Format:	Journal Article
Language:	Polish
Published:	Poland 2007
Subjects:	Carrier Proteins - genetics Carrier Proteins - metabolism Child Diarrhea - microbiology Escherichia coli - classification Escherichia coli - enzymology Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Humans Phylogeny Poland Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length - genetics Serine Endopeptidases - chemistry Serine Endopeptidases - genetics Serine Endopeptidases - metabolism Virulence Factors - chemistry Virulence Factors - genetics Virulence Factors - metabolism Poland
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Summary:	The group of 96 strains ofEscherichia coli isolated from children with diarrhea were investigated towards the presence and polymorphism of genes encoding autotransporters that belong to the group of proteins named SPATE (Serine Protease Autotransporters ofEnterobacteriaceae). Based on the results of restriction analysis of the products of PCR reaction 8 different types of genes encoding SPATE were detected. It was found that 39 strains contained one gene of SPATE, 15 strains contained two different genes and 3 different genes were detected in the case of 3 strains. The analysis of combination of presence of genes encoding SPATE let us divide the investigated group of strains into 17 different genotypes. The analysis of polymorphism of genes encoding SPATE seems to be very promising tool for exploring the genetic diversity among pathogenic E. coli.
ISSN:	0025-8601