Search Results - "Ziomek, Carol"

Transgenically Produced Human Antithrombin: Structural and Functional Comparison to Human Plasma–Derived Antithrombin by Edmunds, Tim, Van Patten, Scott M., Pollock, Julie, Hanson, Eric, Bernasconi, Richard, Higgins, Elizabeth, Manavalan, Partha, Ziomek, Carol, Meade, Harry, McPherson, John M., Cole, Edward S.

“…Recombinant human antithrombin (rhAT) produced in transgenic goat milk was purified to greater than 99%. The specific activity of the rhAT was identical to…”
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Effect of serum concentration, method of trypsinization and fusion/activation utilizing transfected fetal cells to generate transgenic dairy goats by somatic cell nuclear transfer by Melican, David, Butler, Robin, Hawkins, Nathan, Chen, Li-How, Hayden, Evelyn, Destrempes, Margaret, Williams, Jennifer, Lewis, Tanya, Behboodi, Esmail, Ziomek, Carol, Meade, Harry, Echelard, Yann, Gavin, William

“…This work was performed within a commercial nuclear transfer program to investigate different methods for synchronizing donor cell cycle stage, for harvesting…”
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Production of goats by somatic cell nuclear transfer by Baguisi, A, Behboodi, E, Melican, D.T, Pollock, J.S, Destrempes, M.M, Cammuso, C, Williams, J.L, Nims, S.D, Porter, C.A, Midura, P

“…In this study, we demonstrate the production of transgenic goats by nuclear transfer of fetal somatic cells. Donor karyoplasts were obtained from a primary…”
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Two alkaline phosphatase genes are expressed during early development in the mouse embryo by Hahnel, A C, Rappolee, D A, Millan, J L, Manes, T, Ziomek, C A, Theodosiou, N G, Werb, Z, Pedersen, R A, Schultz, G A

“…Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell…”
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Genomic structure and comparison of mouse tissue-specific alkaline phosphatase genes by Manes, T, Glade, K, Ziomek, C A, Millán, J L

“…A full-length human placental alkaline phosphatase (AP) cDNA was used to identify and clone related genes from mouse genomic libraries. We report the cloning,…”
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One-minute exposure of 4-cell mouse embryos to glucose overcomes morula block in CZB medium by Chatot, C L, Lewis-Williams, J, Torres, I, Ziomek, C A

“…One-cell mouse embryos that block at the 2-cell stage can progress to the morula stage in CZB medium, but fail to cavitate and then swell and lyse. A 1-min…”
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Embryonic alkaline phosphatase is expressed at M-phase in the spermatogenic lineage of the mouse by NARISAWA, S, HOFMANN, M.-C, ZIOMEK, C. A, MILLAN, J. L

“…We have recently cloned and characterized a novel embryonic alkaline phosphatase (EAP) expressed at the two-cell to blastocyst stage of preimplantation…”
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No Immunogenicity Found to Antithrombin alfa in Human Clinical Studies by Frieling, Johan, Ziomek, Carol, Masiello, Nick, Peters, Lynn, Richards, Susan, Welch, Karen, Theobald, Valerie, Bonfiglio, Judith, Chen, LiHow, Meade, Harry

“…Antithrombin alfa (AT alfa or recombinant human AT) became the first approved medicine produced in the milk of transgenic goats, when EMEA granted it marketing…”
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Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer by Jellerette, Teru, Melican, David, Butler, Robin, Nims, Scott, Ziomek, Carol, Fissore, Rafael, Gavin, William

“…Routine activation of nuclear transfer (NT) eggs involves the application of a single intracellular calcium [Ca 2+] i rise, stimulated by an electrical pulse,…”
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Cell interactions influence the fate of mouse blastomeres undergoing the transition from the 16- to the 32-cell stage by Johnson, Martin H., Ziomek, Carol Ann

“…Newly formed polar and apolar 1 16 blastomeres were isolated and cultured singly, or in various combinations, through division to form 32-cell blastomeres. The…”
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The roles of phenotype and position in guiding the fate of 16-cell mouse blastomeres by Ziomek, Carol A., Johnson, Martin H.

“…Newly formed 16-cell blastomeres were typed as larger or smaller, labelled with the short-term lineage marker FITC, and aggregated in various spatial arrays…”
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Differential regulation of G protein subunit expression in mouse oocytes, eggs, and early embryos by Allworth, Ann E., Hildebrandt, John D., Ziomek, Carol A.

“…Pertussis toxin ADP-ribosylation and Western blot analysis using G protein-specific antibodies were used to study G protein expression in mouse oocytes, eggs,…”
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Production of recombinant therapeutic proteins in the milk of transgenic animals by Echelard, Yann, Ziomek, Carol A, Meade, Harry M

“…Transgenic milk production offers a cost-effective system for the manufacturing of large amounts of complex proteins. Specifically, commercialization is near…”
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Recombinant antithrombin: production and role in cardiovascular disorder by Levy, J H, Weisinger, A, Ziomek, C A, Echelard, Y

“…Plasma-derived antithrombin (AT) concentrates have been used for the management of hereditary and acquired deficiencies since the early 1980s. Recombinant…”
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Urine as a substitute for milk? by Meade, Harry, Ziomek, Carol

“…In the early days of biopharming, three body fluids - milk, blood, and urine - were proposed for transgenic protein production and, over the past 10 years,…”
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The foundation of two distinct cell lineages within the mouse morula by Johnson, M H, Ziomek, C A

“…The division of single cells, isolated from an 8-cell mouse embryo, to give 2 x 1/16 cells has been studied by sampling cells for analysis at defined stages…”
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Transgenically Produced Human Antithrombin: Structural and Functional Comparison to Human Plasma–Derived Antithrombin by Edmunds, Tim, Van Patten, Scott M., Pollock, Julie, Hanson, Eric, Bernasconi, Richard, Higgins, Elizabeth, Manavalan, Partha, Ziomek, Carol, Meade, Harry, McPherson, John M., Cole, Edward S.

“…Recombinant human antithrombin (rhAT) produced in transgenic goat milk was purified to greater than 99%. The specific activity of the rhAT was identical to…”
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Preimplantation Mouse Embryos Express a Heat-Stable Alkaline Phosphatase1 by Lepire, Merry L., Ziomek, Carol Ann

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Analysis of glutaminase activity and RNA expression in preimplantation mouse embryos by Chatot, Clare L., Lawry, John R., Germain, Bonnie, Ziomek, Carol A.

“…Glutamine is utilized as an energy substrate in preimplantation mouse embryos. Glutaminase is the enzyme responsible for the conversion of glutamine to…”
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Redistribution of Membrane Proteins in Isolated Mouse Intestinal Epithelial Cells by Ziomek, Carol Ann, Schulman, Seth, Edidin, Michael

“…Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species…”
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