A Pyrimidine Auxotroph of Sclerotinia sclerotiomm for Use in Biological Weed Control
Department of Plant Pathology, Montana State University, Bozeman, Montana 59717-0002, USA ABSTRACT SUMMARY: An auxotroph of Sclerotinia sclerotiomm , A1-pyr, was isolated after mutagenesis of ascospores with ultraviolet light. Addition of cytosine and to a lesser extent uracil, but not thymidine, to...
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| Published in: | Journal of general microbiology Vol. 135; no. 7; pp. 2085 - 2091 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Soc General Microbiol
01-07-1989
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| Online Access: | Get full text |
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| Summary: | Department of Plant Pathology, Montana State University, Bozeman, Montana 59717-0002, USA
ABSTRACT
SUMMARY: An auxotroph of Sclerotinia sclerotiomm , A1-pyr, was isolated after mutagenesis of ascospores with ultraviolet light. Addition of cytosine and to a lesser extent uracil, but not thymidine, to minimal medium permitted growth of this auxotroph. Protoplasts of Al-pyr failed to regenerate on Czapek agar medium without cytosine supplementation (20 mg 1 -1 ); on cytosine-supplemented medium 01% of protoplasts successfully regenerated. The mutant was avirulent on four of seven susceptible hosts unless an exogenous cytosine source was applied at the inoculation site. The requirement for external cytosine makes A1-pyr a potential candidate for use in biological weed control. While isolates of this fungus obtained from nature attack numerous beneficial crop and native plants, A1-pyr would be limited to the area of cytosine application, thus reducing the threat to beneficial plants.
Present address: Mycogen Corporation, 3303 McDonald Avenue, Ruston, Louisiana 71270, USA.
Present address: Department of Plant Pathology, 139 Funchess Hall, Auburn University, Auburn, Alabama 36849-5409, USA. |
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| ISSN: | 0022-1287 1350-0872 1465-2080 |
| DOI: | 10.1099/00221287-135-7-2085 |