Diet influences knee osteoarthritis osteophyte formation via gut microbiota and serum metabolites

Osteophyte formation, a key indicator of osteoarthritis (OA) severity, remains poorly understood in its relation to gut microbiota and metabolites in knee osteoarthritis (KOA). We conducted 16S rDNA sequencing and untargeted metabolomics on fecal and serum samples from 20 healthy volunteers, 80 KOA...

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Bibliographic Details
Published in:	iScience Vol. 27; no. 6; p. 110111
Main Authors:	Zhu, Dandan, Wang, Xiaochao, Xi, Zhihui, Chen, Kunling, Feng, Yatian, Zi, Chunjian, Pan, Zhijian, Ma, Xinyu, Zeng, Xiaolong, Feng, Huolun, Guo, Da
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 21-06-2024 Elsevier
Subjects:	Health sciences Metabolomics Microbiome Orthopedics Health sciences Metabolomics Orthopedics Microbiome
Online Access:	Get full text
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Summary:	Osteophyte formation, a key indicator of osteoarthritis (OA) severity, remains poorly understood in its relation to gut microbiota and metabolites in knee osteoarthritis (KOA). We conducted 16S rDNA sequencing and untargeted metabolomics on fecal and serum samples from 20 healthy volunteers, 80 KOA patients in Guangdong, and 100 in Inner Mongolia, respectively. Through bioinformatics analysis, we identified 3 genera and 5 serum metabolites associated with KOA osteophyte formation. Blautia abundance negatively correlated with meat, cheese, and bean consumption. The 5 serum metabolites negatively correlated with dairy, beef, cheese, sugar, and salt intake, yet positively with age and oil consumption. Higher Blautia levels in the gut may contribute to KOA osteophyte formation, with serum metabolites LTB4 and PGD2 potentially serving as biomarkers. KOA patients in Inner Mongolia exhibited lower Blautia levels and reduced expression of 5 serum metabolites, possibly due to cheese consumption habits, resulting in less osteophyte formation. [Display omitted] •Higher Blautia levels in gut microbiota may contribute to osteophyte formation in KOA•LTB4 and PGD2 may serve as biomarkers linked to osteophyte formation in KOA•Five metabolites，fecal Blautia levels negatively correlated with cheese consumption Health sciences; Orthopedics; Microbiome; Metabolomics
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally Lead contact
ISSN:	2589-0042 2589-0042
DOI:	10.1016/j.isci.2024.110111