The role of the yolk sac in copper metabolism during rat embryogenesis

Using the immunoblotting method, the synthesis of two copper-transporting P1-type ATPases, ATP7A (a candidate for the product of the Menkes disease gene) and ATP7B (presumed product of the Wilson disease gene), in the yolk sac cells of rat embryos at days 11 and 20 of embryogenesis was demonstrated....

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Bibliographic Details
Published in:	Ontogenez Vol. 32; no. 3; p. 204
Main Authors:	Puchkova, L V, Zhiguleva, C A, Mokshina, S V, Bichevaia, N K, Platonova, N A, Skvortsova, N N, Sasina, L K, Tsymbalenko, N V, Chebotar', N A, Gaitshoki, V S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-2001
Subjects:	Adenosine Triphosphatases - metabolism Amino Acid Sequence Animals Carrier Proteins - metabolism Cation Transport Proteins - metabolism Ceruloplasmin - metabolism Copper - metabolism Copper-transporting ATPases Female Molecular Sequence Data Pregnancy Rats Recombinant Fusion Proteins Yolk Sac - metabolism
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Summary:	Using the immunoblotting method, the synthesis of two copper-transporting P1-type ATPases, ATP7A (a candidate for the product of the Menkes disease gene) and ATP7B (presumed product of the Wilson disease gene), in the yolk sac cells of rat embryos at days 11 and 20 of embryogenesis was demonstrated. Concomitantly, yolk sac cells produce ceruloplasmin, a soluble copper-transporting glycoprotein, a proportion of which in secreted proteins progressively diminishes, attaining 5.2% at day 11 and 3.1% at day 20 of development. At different stages of embryogenesis, yolk sac cells synthesize two molecular forms of [14]C-ceruloplasmin, one of which is secreted towards the embryo, whereas the other, towards the decidual membrane. Two forms of ceruloplasmin secreted in polar directions differ in the rate of secretion. The role of the yolk sac as a key organ controlling the delivery and secretion of copper in the embryo during the postimplantation period is discussed.
ISSN:	0475-1450