Genomic Landscape and Regulation of RNA Editing in Pekin Ducks Susceptible to Duck Hepatitis A Virus Genotype 3 Infection

RNA editing is increasingly recognized as a post-transcriptional modification that directly affects viral infection by regulating RNA stability and recoding proteins. the duck hepatitis A virus genotype 3 (DHAV-3) infection is seriously detrimental to the Asian duck industry. However, the landscape...

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Bibliographic Details
Published in:	International journal of molecular sciences Vol. 25; no. 19; p. 10413
Main Authors:	Zhao, Haonao, Wu, Zifang, Wang, Zezhong, Ru, Jinlong, Wang, Shuaiqin, Li, Yang, Hou, Shuisheng, Zhang, Yunsheng, Wang, Xia
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-10-2024 MDPI
Subjects:	3' Untranslated Regions - genetics Adenosine Animals Development and progression DHAV-3 Disease Susceptibility Diseases Ducks Ducks - genetics Ducks - virology Editing Enzymes Gene expression Genetic aspects Genomes Genotype Health aspects Hepatitis Hepatitis A Hepatitis Virus, Duck - genetics Hepatitis, Viral, Animal - genetics Hepatitis, Viral, Animal - virology Infections Liver - metabolism Liver - virology MicroRNAs MicroRNAs - genetics miRNA Pekin ducks Physiological aspects Picornaviridae Infections - genetics Picornaviridae Infections - veterinary Picornaviridae Infections - virology Poultry Poultry Diseases - genetics Poultry Diseases - virology Proteins Risk factors RNA Editing RNA processing susceptibility Viral infections China DHAV-3 miRNA Pekin ducks RNA editing susceptibility
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Summary:	RNA editing is increasingly recognized as a post-transcriptional modification that directly affects viral infection by regulating RNA stability and recoding proteins. the duck hepatitis A virus genotype 3 (DHAV-3) infection is seriously detrimental to the Asian duck industry. However, the landscape and roles of RNA editing in the susceptibility and resistance of Pekin ducks to DHAV-3 remain unclear. Here, we profiled dynamic RNA editing events in liver tissue and investigated their potential functions during DHAV-3 infection in Pekin ducks. We identified 11,067 informative RNA editing sites in liver tissue from DHAV-3-susceptible and -resistant ducklings at three time points during virus infection. Differential RNA editing sites (DRESs) between S and R ducks were dynamically changed during infection, which were enriched in genes associated with vesicle-mediated transport and immune-related pathways. Moreover, we predicted and experimentally verified that RNA editing events in 3'-UTR could result in loss or gain of miRNA-mRNA interactions, thereby changing the expression of target genes. We also found a few DRESs in coding sequences (CDSs) that altered the amino acid sequences of several proteins that were vital for viral infection. Taken together, these data suggest that dynamic RNA editing has significant potential to tune physiological processes in response to virus infection in Pekin ducks, thus contributing to host differential susceptibility to DHAV-3.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms251910413