Expressed sequence tags from Thellungiella halophila, a new model to study plant salt-tolerance

Accumulation and analysis of expressed sequence tags (ESTs) data from halophytic plant is a relatively rapid and cheap way for discovering new genes related to salinity tolerance. We constructed a NaCl-treated cDNA library of Thellungiella halophila and sequenced more than 1500 randomly selected clo...

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Bibliographic Details
Published in:	Plant science (Limerick) Vol. 166; no. 3; pp. 609 - 616
Main Authors:	Wang, Zeng-lan, Li, Ping-hua, Fredricksen, Mark, Gong, Zhi-zhong, Kim, C.S, Zhang, Changquing, Bohnert, Hans J, Zhu, Jian-Kang, Bressan, Ray A, Hasegawa, Paul M, Zhao, Yan-xiu, Zhang, Hui
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier Ireland Ltd 01-03-2004 Elsevier Science
Subjects:	Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions Biological and medical sciences Brassicaceae cDNA library complementary DNA EST expressed sequence tags Fundamental and applied biological sciences. Psychology gene expression regulation Genetics and breeding of economic plants Halophyte halophytes Northern blotting plant genetics Plant physiology and development plant proteins salinity salt tolerance Thellungiella halophia Thellungiella halophila Thellungiella salsuginea Varietal selection. Specialized plant breeding, plant breeding aims Water and solutes. Absorption, translocation and permeability Halophyte Thellungiella halophila EST cDNA library cDNA lihrary Genetic selection Salt resistance Genetic marker Interspecific comparison Tolerance Identification Salinity Arabidopsis thaliana Gene Investigation method Cruciferae Dicotyledones Angiospermae Spermatophyta Experimental plant Expressed sequence tag Thellmigiella hohophila
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Summary:	Accumulation and analysis of expressed sequence tags (ESTs) data from halophytic plant is a relatively rapid and cheap way for discovering new genes related to salinity tolerance. We constructed a NaCl-treated cDNA library of Thellungiella halophila and sequenced more than 1500 randomly selected clones. By sequence analysis, 813 unique clones were identified: 549 showed homology to previously identified genes, 264 matched uncharacterized genes. All our EST data are available on the Internet. The identity between T. halophila and Arabidopsis thaliana cDNA sequences in our EST collection are 95.76% in total ESTs and 95.36% in non-redundant clones. At least eight classes of genes were related to the salt-tolerance, which accounted for about 18.89% of total sequenced ESTs. T. halophila that has many features similar to Arabidopsis can be adopted as a halophytic model for stress-tolerance research.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0168-9452 1873-2259
DOI:	10.1016/j.plantsci.2003.10.030