Potential of using medicinal plant extracts as photosensitizers for antimicrobial photodynamic therapy

Potential of using medicinal plant extracts as photosensitizers for antimicrobial photodynamic therapy

Antimicrobial photodynamic therapy (APDT) is a promising approach to overcome antimicrobial resistance. However, for widespread implementation of this approach, approved photosensitizers are needed. In this study, we used commercially available preparations (Calendulae officinalis floridis extract,...

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Published in:Photochemistry and photobiology Vol. 100; no. 6; pp. 1833 - 1847
Main Authors: Mikulich, Aliaksandr V, Plavskii, Vitaly Yu, Tretyakova, Antonina I, Nahorny, Raman K, Sobchuk, Andrey N, Dudchik, Natalia V, Emeliyanova, Olga A, Zhabrouskaya, Anastasia I, Plavskaya, Ludmila G, Ananich, Tatsiana S, Dudinova, Olga N, Leusenka, Ihar A, Yakimchuk, Sergey V, Svechko, Alexei D, Tien, Tran Quoc, Tong, Quang Cong, Nguyen, Thanh Phuong
Format: Journal Article
Language:English
Published: United States Blackwell Publishing Ltd 01-11-2024
Subjects:
Antimicrobial agents
Antimicrobial resistance
Bacteria
Chlorophyll
Chromophores
Eucalyptus
Herbal medicine
Hypericin
Inactivation
Irradiance
Irradiation
Light effects
Medicinal plants
Methylene blue
Photodynamic therapy
Plant extracts
Pseudomonas aeruginosa
Radiation
Singlet oxygen
Wavelengths
medicinal plant extracts
photosensitizers
singlet oxygen
antimicrobial resistance
gram-positive and gram-negative bacteria
antimicrobial photodynamic therapy
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Summary:Antimicrobial photodynamic therapy (APDT) is a promising approach to overcome antimicrobial resistance. However, for widespread implementation of this approach, approved photosensitizers are needed. In this study, we used commercially available preparations (Calendulae officinalis floridis extract, Chamomillae recutitae floridis extract, Achillea millefolii herbae extract; Hypericum perforatum extract; Eucalyptus viminalis folia extract) as photosensitizers for inactivation of gram-negative (Pseudomonas aeruginosa) and gram-positive (Staphylococcus aureus) bacteria. Spectral-luminescent analysis has shown that the major chromophores are of chlorophyll (mainly chlorophyll a and b) and hypericin nature. The extracts are efficient generators of singlet oxygen with quantum yield (γ ) from 0.40 to 0.64 (reference compound, methylene blue with γ = 0.52). In APDT assays, bacteria before irradiation were incubated with extracts for 30 min. After irradiation and 24 h of incubation, colony-forming units (CFU) were counted. Upon exposure of P. aeruginosa to radiation of 405 nm, 590 nm, and 660 nm at equal energy dose of 30 J/cm (irradiance - 100 mW/cm , exposure time - 5 min), the most pronounced effect is observed with blue light (>3 log reduction); in case of S. aureus, the effect is approximately equivalent for light of indicated wavelengths and dose (>4 log reduction).
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content type line 23
ISSN:0031-8655
1751-1097
1751-1097
DOI:10.1111/php.13935