Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes

Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes

•Glucose and lactate sensors with wide measurement ranges up to 600/900mM.•Study of polyurethane membranes: morphology, permeability, sensor performance.•Evaluation of sensor sterilization using gamma and e-beam irradiation.•In-situ glucose monitoring in a yeast culture over 13days.•Long-term study...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Vol. 241; pp. 48 - 54
Main Authors: Mross, Stefan, Zimmermann, Tom, Zenzes, Sebastian, Kraft, Michael, Vogt, Holger
Format: Journal Article Web Resource
Language:English
Published: Lausanne Elsevier B.V 31-03-2017
Elsevier Science Ltd
Elsevier Sequoia
Subjects:
Baking yeast
Bioprocess monitoring
Biotechnological process
Biotechnology
Electrical & electronics engineering
Electron beam irradiation
Electron beams
Electron irradiation
Engineering, computing & technology
Enzyme sensors
Enzymes
Fabrication parameters
Glucose
Immunoglobulins
In- situ monitoring
Ingénierie électrique & électronique
Ingénierie, informatique & technologie
Long term performance
Membranes
Monitoring
Morphology
Performance evaluation
Polymer concentrations
Polyurethane membrane
Polyurethane membranes
Polyurethane resins
Polyurethanes
Protein expression
Rangefinding
Saccharomyces cerevisiae
Sensors
Solvents
Surface morphology
Tetrahydrofuran
Yeast
Polyurethane membrane
Enzyme sensors
In-situ monitoring
Surface morphology
Bioprocess monitoring
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Summary:•Glucose and lactate sensors with wide measurement ranges up to 600/900mM.•Study of polyurethane membranes: morphology, permeability, sensor performance.•Evaluation of sensor sterilization using gamma and e-beam irradiation.•In-situ glucose monitoring in a yeast culture over 13days.•Long-term study of sensor behavior over 28days. We report on a study of enzymatic glucose and lactate sensors for measurements of wide concentration ranges from 1mM up to 600mM and 900mM, respectively, in biotechnological processes. Diffusion-limiting polyurethane membranes were used to extend the linear measurement range and the influence of two fabrication parameters on membrane properties and sensor performance evaluated. The polymer concentration was varied between 4% and 10% and the ratio of the solvents tetrahydrofuran and dimethylformamide between 9:1 and 1:9. Surface morphology and permeability of the membranes and the linear measurement range of membrane-covered enzyme sensors were determined. A distinct relationship between the different results was found. The sensors retained their functionality after being sterilized using gamma and electron beam irradiation. The long-term performance of the sensors was evaluated and good performance in the glucose monitoring of a culture of Saccharomyces cerevisiae over 13days was shown.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
scopus-id:2-s2.0-84991706319
ISSN:0925-4005
1873-3077
1873-3077
DOI:10.1016/j.snb.2016.10.054