Search Results - "Zel, Jana"

Quantitative analysis of food and feed samples with droplet digital PCR by Morisset, Dany, Štebih, Dejan, Milavec, Mojca, Gruden, Kristina, Žel, Jana

“…In this study, the applicability of droplet digital PCR (ddPCR) for routine analysis in food and feed samples was demonstrated with the quantification of…”
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Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection by Dobnik, David, Štebih, Dejan, Blejec, Andrej, Morisset, Dany, Žel, Jana

“…The advantages of the digital PCR technology are already well documented until now. One way to achieve better cost efficiency of the technique is to use it in…”
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Digital PCR as an effective tool for GMO quantification in complex matrices by Bogožalec Košir, Alexandra, Demšar, Tina, Štebih, Dejan, Žel, Jana, Milavec, Mojca

“…[Display omitted] •Droplet digital PCR (ddPCR) and chamber-based dPCR (cdPCR) were used to quantify GMO levels.•GMO assays targeted soybean reference lectin…”
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Assessment of the real-time PCR and different digital PCR platforms for DNA quantification by Pavšič, Jernej, Žel, Jana, Milavec, Mojca

“…Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. Several analytical…”
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Optimising droplet digital PCR analysis approaches for detection and quantification of bacteria: a case study of fire blight and potato brown rot by Dreo, Tanja, Pirc, Manca, Ramšak, Živa, Pavšič, Jernej, Milavec, Mojca, Žel, Jana, Gruden, Kristina

“…Here we report on the first assessment of droplet digital PCR (ddPCR) for detection and absolute quantification of two quarantine plant pathogenic bacteria…”
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Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR by Košir, Alexandra Bogožalec, Divieto, Carla, Pavšič, Jernej, Pavarelli, Stefano, Dobnik, David, Dreo, Tanja, Bellotti, Roberto, Sassi, Maria Paola, Žel, Jana

“…Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic decisions, as overestimation or underestimation can lead to misguided…”
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Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines by Košir, Alexandra Bogožalec, Spilsberg, Bjørn, Holst-Jensen, Arne, Žel, Jana, Dobnik, David

“…Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds…”
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Digital PCR for direct quantification of viruses without DNA extraction by Pavšič, Jernej, Žel, Jana, Milavec, Mojca

“…DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly…”
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GMO quantification: valuable experience and insights for the future by Milavec, Mojca, Dobnik, David, Yang, Litao, Zhang, Dabing, Gruden, Kristina, Žel, Jana

“…Cultivation and marketing of genetically modified organisms (GMOs) have been unevenly adopted worldwide. To facilitate international trade and to provide…”
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New approaches in GMO detection by Querci, Maddalena, Van den Bulcke, Marc, Žel, Jana, Van den Eede, Guy, Broll, Hermann

“…The steady rate of development and diffusion of genetically modified plants and their increasing diversification of characteristics, genes and genetic control…”
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Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products by Holst-Jensen, Arne, Spilsberg, Bjørn, Arulandhu, Alfred J., Kok, Esther, Shi, Jianxin, Zel, Jana

“…The emergence of high-throughput, massive or next-generation sequencing technologies has created a completely new foundation for molecular analyses. Various…”
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Involvement of potato (Solanum tuberosum L.) MKK6 in response to potato virus Y by Lazar, Ana, Coll, Anna, Dobnik, David, Baebler, Spela, Bedina-Zavec, Apolonija, Zel, Jana, Gruden, Kristina

“…Mitogen-activated protein kinase (MAPK) cascades have crucial roles in the regulation of plant development and in plant responses to stress. Plant recognition…”
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GMO matrix: A cost-effective approach for screening unauthorized genetically modified events in India by Randhawa, Gurinder Jit, Morisset, Dany, Singh, Monika, Žel, Jana

“…Use of a pragmatic, affordable and reliable approach for screening and detection of a large number of genetically modified (GM) crops/events is the need of…”
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Inter-laboratory analysis of selected genetically modified plant reference materials with digital PCR by Dobnik, David, Demšar, Tina, Huber, Ingrid, Gerdes, Lars, Broeders, Sylvia, Roosens, Nancy, Debode, Frederic, Berben, Gilbert, Žel, Jana

“…Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism (GMO) testing, enables determination of absolute target copy…”
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GMOseek: a user friendly tool for optimized GMO testing by Morisset, Dany, Novak, Petra Kralj, Zupanič, Darko, Gruden, Kristina, Lavrač, Nada, Žel, Jana

“…With the increasing pace of new Genetically Modified Organisms (GMOs) authorized or in pipeline for commercialization worldwide, the task of the laboratories…”
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Insertion of a specific fungal 3'-phosphoadenosine-5'-phosphatase motif into a plant homologue improves halotolerance and drought tolerance of plants by Gašparič, Meti Buh, Lenassi, Metka, Gostinčar, Cene, Rotter, Ana, Plemenitaš, Ana, Gunde-Cimerman, Nina, Gruden, Kristina, Zel, Jana

“…Soil salinity and drought are among the most serious agricultural and environmental problems of today. Therefore, investigations of plant resistance to abiotic…”
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Decision Support for the Comparative Evaluation and Selection of Analytical Methods: Detection of Genetically Modified Organisms as an Example by Dobnik, David, Gruden, Kristina, Žel, Jana, Bertheau, Yves, Holst-Jensen, Arne, Bohanec, Marko

“…The selection of the best-fit-for-purpose analytical method to be implemented in the laboratory is difficult due to availability of multiple methods, targets,…”
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Multiplex Quantification of 12 European Union Authorized Genetically Modified Maize Lines with Droplet Digital Polymerase Chain Reaction by Dobnik, David, Spilsberg, Bjørn, Bogožalec Košir, Alexandra, Holst-Jensen, Arne, Žel, Jana

“…Presence of genetically modified organisms (GMO) in food and feed products is regulated in many countries. The European Union (EU) has implemented a threshold…”
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Loop-Mediated Isothermal Amplification: Rapid Visual and Real-Time Methods for Detection of Genetically Modified Crops by Randhawa, Gurinder Jit, Singh, Monika, Morisset, Dany, Sood, Payal, Žel, Jana

“…A rapid, reliable, and sensitive loop-mediated isothermal amplification (LAMP) system was developed for screening of genetically modified organisms (GMOs). The…”
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Comparison of Genome Sequence of PVY Isolates with Biological Properties by Barker, Hugh, McGeachy, Kara D, Toplak, Nataša, Gruden, Kristina, Žel, Jana, Browning, Isla

“…The whole RNA genomes of six European Potato virus Y (PVY) isolates, representing isolates of the PVY O , PVY N and PVY NTN subgroups, were sequenced and the…”
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