Search Results - "Zawada, James F."
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Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines
Published in Biotechnology and bioengineering (01-07-2011)“…Engineering robust protein production and purification of correctly folded biotherapeutic proteins in cell‐based systems is often challenging due to the…”
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Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis
Published in Biotechnology progress (01-03-2005)“…Escherichia coli extracts activate cell‐free protein synthesis systems by providing the catalysts for translation and other supporting reactions. Recent…”
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Cell-free technologies for biopharmaceutical research and production
Published in Current opinion in biotechnology (01-08-2022)“…Cell-free protein synthesis (CFPS) technologies have grown from lab-scale research tools to biopharmaceutical production at the Good Manufacturing Practice…”
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A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems
Published in Biotechnology progress (01-05-2015)“…Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding…”
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Preparation and testing of E. coli S30 in vitro transcription translation extracts
Published in Methods in molecular biology (Clifton, N.J.) (2012)“…Crude cell-free extracts are useful tools for investigating biochemical phenomena and exploiting complex enzymatic processes such as protein synthesis…”
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A simplified and robust protocol for immunoglobulin expression in E scherichia coli cell‐free protein synthesis systems
Published in Biotechnology progress (01-05-2015)“…Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding…”
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Journal Article