Search Results - "Zawada, James"

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines by Zawada, James F., Yin, Gang, Steiner, Alexander R., Yang, Junhao, Naresh, Alpana, Roy, Sushmita M., Gold, Daniel S., Heinsohn, Henry G., Murray, Christopher J.

“…Engineering robust protein production and purification of correctly folded biotherapeutic proteins in cell‐based systems is often challenging due to the…”
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Cell-free technologies for biopharmaceutical research and production by Zawada, James F, Burgenson, David, Yin, Gang, Hallam, Trevor J, Swartz, James R, Kiss, Robert D

“…Cell-free protein synthesis (CFPS) technologies have grown from lab-scale research tools to biopharmaceutical production at the Good Manufacturing Practice…”
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A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems by Cai, Qi, Hanson, Jeffrey A., Steiner, Alexander R., Tran, Cuong, Masikat, Mary Rose, Chen, Rishard, Zawada, James F., Sato, Aaron K., Hallam, Trevor J., Yin, Gang

“…Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding…”
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Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system by Yin, Gang, Garces, Eudean D., Yang, Junhao, Zhang, Juan, Tran, Cuong, Steiner, Alexander R, Roos, Christine, Bajad, Sunil, Hudak, Susan, Penta, Kalyani, Zawada, James, Pollitt, Sonia, Murray, Christopher J.

“…We describe protein synthesis, folding and assembly of antibody fragments and full-length aglycosylated antibodies using an Escherichia coli-based open…”
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An Integrated In Vivo/In Vitro Protein Production Platform for Site-Specific Antibody Drug Conjugates by Hanson, Jeffrey, Groff, Dan, Carlos, Abi, Usman, Hans, Fong, Kevin, Yu, Abigail, Armstrong, Stephanie, Dwyer, Allison, Masikat, Mary Rose, Yuan, Dawei, Tran, Cuong, Heibeck, Tyler, Zawada, James, Chen, Rishard, Hallam, Trevor, Yin, Gang

“…The XpressCF+ cell-free protein synthesis system is a robust platform for the production of non-natural amino acids containing antibodies, which enable the…”
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Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis by Liu, David V., Zawada, James F., Swartz, James R.

“…Escherichia coli extracts activate cell‐free protein synthesis systems by providing the catalysts for translation and other supporting reactions. Recent…”
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Maintaining rapid growth in moderate-density Escherichia coli fermentations by Zawada, James, Swartz, James

“…A novel feeding strategy that prolongs rapid growth rates for Escherichia coli fermentations to moderately high cell density is presented. High‐density…”
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Effects of growth rate on cell extract performance in cell-free protein synthesis by Zawada, James, Swartz, James

“…Cell‐free protein synthesis is a useful research tool and now stands poised to compete with in vivo expression for commercial production of proteins. However,…”
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Preparation and testing of E. coli S30 in vitro transcription translation extracts by Zawada, James F

“…Crude cell-free extracts are useful tools for investigating biochemical phenomena and exploiting complex enzymatic processes such as protein synthesis…”
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Abstract 742: Nonclinical safety evaluation of STRO-001, a site-specific anti-CD74 antibody-drug conjugate for the potential treatment of B-cell malignancies by Solis, Willy, Almeida, Venita De, Yu, Abigail, Bruhns, Maureen, Zawada, James, Galan, Adam, Matheny, Shannon, Molina, Arturo, Hallam, Trevor, Lupher, Mark

“…Abstract Antibody-drug conjugates (ADCs) constitute an expanding class of therapeutic molecules in preclinical and clinical development for multiple oncology…”
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Abstract NT-090: PRECLINICAL ACTIVITY AND SAFETY OF STRO-002, A NOVEL ADC TARGETING FOLATE RECEPTOR ALPHA FOR OVARIAN AND ENDOMETRIAL CANCER by Abrahams, Cristina, Krimm, Stellanie, Li, Xiaofan, Zhou, Sihong, Hanson, Jeffrey, Masikat, Mary Rose, Bajjuri, Krishna, Heibeck, Tyler, Kothari, Dharti, Yu, Abigail, Henningsen, Robert, Tran, Cuong, Yin, Gang, Zawada, James, Hang, Julie, Bruhns, Maureen, Solis, Willy, Steiner, Alexander, Galan, Adam, Kline, Toni, Stafford, Ryan, Yam, Alice, Almeida, Venita I. De, Lupher, Mark, Hallam, Trevor

“…Abstract OBJECTIVES: Folate receptor alpha (FolRα) is a cell-surface glycoprotein, highly expressed in ovarian and endometrial adenocarcinoma, and thus a…”
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Abstract 67: Characterization and preclinical development of STRO-001, a novel CD74-targeting antibody-drug conjugate (ADC) for the treatment of B-cell malignancies by Abrahams, Cristina, Li, Xiaofan, DeAlmeida, Venita, Embry, Millicent, Yu, Abigail, Krim, Stellanie, Hoffmann, Heidi, Zawada, James, Bruhns, Maureen, Matheny, Shannon, Bussell, Stuart, Kline, Toni, Yam, Alice, Stafford, Ryan, Hallam, Trevor, Lupher, Mark, Molina, Arturo

“…Abstract CD74 is a type II transmembrane glycoprotein involved in the formation and transport of MHC class II protein. CD74 is highly expressed in many B-cell…”
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Abstract 1782: Discovery and activity of STRO-002, a novel ADC targeting folate receptor alpha for ovarian and endometrial cancer by Li, Xiaofan, Abrahams, Cristina, Zhou, Sihong, Krimm, Stellanie, Henningsen, Robert, Stephenson, Heather, Hanson, Jeffrey, Masikat, Mary Rose, Bajjuri, Krishna, Heibeck, Tyler, Tran, Cuong, Yin, Gang, Zawada, James, Sarma, Ganapathy, Chen, Joy, Bruhns, Maureen, Solis, Willy, Steiner, Alexander, Galan, Adam, Kline, Toni, Stafford, Ryan, Yam, Alice, Almeida, Venita I. De, Lupher, Mark, Hallam, Trevor

“…Abstract Folate receptor alpha (FolRα) is a glycosylphosphatidylinositol linked cell-surface glycoprotein that is widely expressed in serous and epithelial…”
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Targeting CD74 with Novel Antibody Drug Conjugates (ADCs) for the Treatment of B-Cell Non-Hodgkin's Lymphoma (NHL) by Li, Xiaofan, Abrahams, Cristina, Embry, Millicent, Yu, Abigail, Kahana, Jason, Brown, Michael, Narla, Rama Krishna, Barnes, Leo, Schwartz, Eric, Boylan, John, Zawada, James, Stephenson, Heather, Bruhns, Maureen, Bussell, Stuart, Steiner, Alexander, Galan, Adam, Kline, Toni, Yam, Alice, Stafford, Ryan, Hoffmann, Heidi, Matheny, Shannon, DeAlmeida, Venita, Vasquez, Nicki, Heinsohn, Henry, Sato, Aaron, Molina, Arturo, Hallam, Trevor, Lupher, Mark

“…CD74 is a type II transmembrane glycoprotein involved in the formation and transport of MHC class II protein. High expression of CD74 has been confirmed in…”
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Discovery and Preclinical Development of Novel CD74-Targeting Antibody-Drug Conjugates (ADCs) with Significant Activity in Multiple Myeloma (MM) Cell Lines and Xenograft Models by Abrahams, Cristina, Li, Xiaofan, Yu, Abigail, Krimm, Stellanie, Kahana, Jason, Narla, Rama Krishna, Schwartz, Eric, Boylan, John, Hoffmann, Heidi, Steiner, Alexander, Zawada, James, Stephenson, Heather, Bruhns, Maureen, DeAlmeida, Venita, Matheny, Shannon, Bussell, Stuart, Galan, Adam, Kline, Toni, Vasquez, Nicki, Yam, Alice, Stafford, Ryan, Heinsohn, Henry, Sato, Aaron, Molina, Arturo, Hallam, Trevor, Lupher, Mark

“…CD74, also known as HLA-DR-associated invariant chain, is a type II transmembrane glycoprotein highly expressed in many B-cell malignancies. The limited…”
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A simplified and robust protocol for immunoglobulin expression in E scherichia coli cell‐free protein synthesis systems by Cai, Qi, Hanson, Jeffrey A., Steiner, Alexander R., Tran, Cuong, Masikat, Mary Rose, Chen, Rishard, Zawada, James F., Sato, Aaron K., Hallam, Trevor J., Yin, Gang

“…Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy manipulation of conditions to improve protein yield and folding…”
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Scale-up, optimization, and analysis of Escherichia coli extracts for cell -free protein synthesis by Zawada, James Francis

“…Cell-free protein synthesis systems have been used for decades to study transcription and translation, and they continue to be used to produce proteins for…”
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