Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)
Helicobacter pylori ( ) is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis...
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| Published in: | Electronic physician Vol. 9; no. 12; pp. 6129 - 6134 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Iran
Electronic physician
01-12-2017
Electronic Physician |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Helicobacter pylori (
) is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis of
infection. In this research, the diagnostic values of serum and salivary serology were compared together to use salivary anti-
test as an alternative method in the future.
During this prospective case-control study on patients who were candidates for endoscopy and gastric biopsy from March 2015 to April 2016 in Shahid Beheshti hospital, Kashan, Iran, serum and salivary samples were obtained for measurement of Immunoglobulin G (IgG) antibody levels against
by enzyme-linked immunosorbent assay (ELISA). Histopathology was the gold standard test. Statistical analysis was performed by SPSS software version 16. Statistical tests included Kolmogorov-Smirnov, independent-samples t-test, Chi-square, Mann-Whitney U, Kruskal-Wallis, McNemar and correlation.
Of 123 patients, sixty-one patients (49.6%) were
-positive according to histology. The median levels of anti-
antibodies in serum (p<0.001) and saliva (p<0.001) of
-positive cases were significantly higher than
-negative cases. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and accuracy of serologic tests in serum were 75%, 79%, 3.5, 0.3, 77% and for saliva were 85%, 82%, 4.7, 0.18, 84% respectively.
Diagnostic values of salivary ELISA are comparable to serum ELISA and can be used as an alternative modality for diagnosis of
infection. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 2008-5842 2008-5842 |
| DOI: | 10.19082/6129 |