Search Results - "Zatopek, Kelly M."

Molecular basis for proofreading by the unique exonuclease domain of Family-D DNA polymerases by Betancurt-Anzola, Leonardo, Martínez-Carranza, Markel, Delarue, Marc, Zatopek, Kelly M., Gardner, Andrew F., Sauguet, Ludovic

“…Replicative DNA polymerases duplicate entire genomes at high fidelity. This feature is shared among the three domains of life and is facilitated by their dual…”
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The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity by Zatopek, Kelly M, Burkhart, Brett W, Morgan, Richard D, Gehring, Alexandra M, Scott, Kristin A, Santangelo, Thomas J, Gardner, Andrew F

“…( ), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII. TkoI is…”
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Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates by Gardner, Andrew F, Jackson, Kiserian M, Boyle, Madeleine M, Buss, Jackson A, Potapov, Vladimir, Gehring, Alexandra M, Zatopek, Kelly M, Corrêa, Jr, Ivan R, Ong, Jennifer L, Jack, William E

“…A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name "Therminator DNA…”
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Archaeal DNA replication and repair: new genetic, biophysical and molecular tools for discovering and characterizing enzymes, pathways and mechanisms by Zatopek, Kelly M, Gardner, Andrew F, Kelman, Zvi

“…DNA replication and repair are essential biological processes needed for the survival of all organisms. Although these processes are fundamentally conserved in…”
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Engineering GID4 for use as an N-terminal proline binder via directed evolution by Ikonomova, Svetlana P, Yan, Bo, Sun, Zhiyi, Lyon, Rachel B, Zatopek, Kelly M, Marino, John P, Kelman, Zvi

“…Nucleic acid sequencing technologies have gone through extraordinary advancements in the past several decades, significantly increasing throughput while…”
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Thermococcus kodakarensis TK0353 is a novel AP lyase with a new fold by Caffrey, Paul J, Eckenroth, Brian E, Burkhart, Brett W, Zatopek, Kelly M, McClung, Colleen M, Santangelo, Thomas J, Doublié, Sylvie, Gardner, Andrew F

“…Hyperthermophilic organisms thrive in extreme environments prone to high levels of DNA damage. Growth at high temperature stimulates DNA base hydrolysis…”
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Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases by Zatopek, Kelly M, Alpaslan, Ece, Evans, Thomas C, Sauguet, Ludovic, Gardner, Andrew F

“…Abstract Family D DNA polymerase (PolD) is the essential replicative DNA polymerase for duplication of most archaeal genomes. PolD contains a unique two-barrel…”
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Capillary Electrophoresis-Based Functional Genomics Screening to Discover Novel Archaeal DNA Modifying Enzymes by Zatopek, Kelly M, Fossa, Samantha L, Bilotti, Katharina, Caffrey, Paul J, Chuzel, Léa, Gehring, Alexandra M, Lohman, Gregory J S, Taron, Christopher H, Gardner, Andrew F

“…It has been predicted that 30 to 80% of archaeal genomes remain annotated as hypothetical proteins with no assigned gene function. Further, many archaeal…”
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Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis by Gehring, Alexandra M., Zatopek, Kelly M., Burkhart, Brett W., Potapov, Vladimir, Santangelo, Thomas J., Gardner, Andrew F.

“…•Archaeal GO glycosylase (AGOG) is a major 8-oxo-dG repair enzyme in the hyperthermophilic archaeon Thermococcus kodakarensis.•AGOG is non-essential for…”
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RADAR-seq: A RAre DAmage and Repair sequencing method for detecting DNA damage on a genome-wide scale by Zatopek, Kelly M., Potapov, Vladimir, Maduzia, Lisa L., Alpaslan, Ece, Chen, Lixin, Evans, Thomas C., Ong, Jennifer L., Ettwiller, Laurence M., Gardner, Andrew F.

“…[Display omitted] •RADAR-seq identifies and detects genome-wide DNA damage events.•RADAR-seq mapped genome-wide ribonucleotides in wild-type and…”
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Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication by Liman, Geraldy L S, Lennon, Christopher W, Mandley, Jaylin L, Galyon, Alina M, Zatopek, Kelly M, Gardner, Andrew F, Santangelo, Thomas J

“…Inteins (intervening proteins), mobile genetic elements removed through protein splicing, often interrupt proteins required for DNA replication, recombination,…”
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An archaeal Cas3 protein facilitates rapid recovery from DNA damage by Miezner, Guy, Turgeman-Grott, Israela, Zatopek, Kelly M, Gardner, Andrew F, Reshef, Leah, Choudhary, Deepak K, Alstetter, Martina, Allers, Thorsten, Marchfelder, Anita, Gophna, Uri

“…Abstract CRISPR-Cas systems provide heritable acquired immunity against viruses to archaea and bacteria. Cas3 is a CRISPR-associated protein that is common to…”
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Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis by Gehring, Alexandra M., Zatopek, Kelly M., Burkhart, Brett W., Potapov, Vladimir, Santangelo, Thomas J., Gardner, Andrew F.

“…Not provided…”
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