Interferon-γ stimulates CD14, TLR2 and TLR4 mRNA expression in gingival fibroblasts increasing responsiveness to bacterial challenge

Highlights • IFN-γ induces mRNA expression of CD14, TLR2 and TLR4 in human gingival fibroblasts. • IFN-γ induces expression of membrane-bound CD14 in human gingival fibroblasts. • Pre-incubation with IFN-γ followed by LPS stimulation increases cytokine production. • LPS-induced cytokine production i...

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Bibliographic Details
Published in:	Archives of oral biology Vol. 61; pp. 36 - 43
Main Authors:	Lappin, M.J, Brown, V, Zaric, S.S, Lundy, F.T, Coulter, W.A, Irwin, C.R
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-01-2016
Subjects:	Advanced Basic Science Blotting, Western CD14 Cells, Cultured Dentistry Enzyme-Linked Immunosorbent Assay Escherichia coli - immunology Fibroblast Fibroblasts - drug effects Flow Cytometry Gingiva - cytology Humans IFN-γ Interferon-gamma - pharmacology Lipopolysaccharide Lipopolysaccharide Receptors - metabolism Lipopolysaccharides - pharmacology Polymerase Chain Reaction RNA, Messenger - metabolism Toll-Like Receptor 2 - metabolism Toll-Like Receptor 4 - metabolism Up-Regulation Lipopolysaccharide IFN-γ CD14 Fibroblast
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Summary:	Highlights • IFN-γ induces mRNA expression of CD14, TLR2 and TLR4 in human gingival fibroblasts. • IFN-γ induces expression of membrane-bound CD14 in human gingival fibroblasts. • Pre-incubation with IFN-γ followed by LPS stimulation increases cytokine production. • LPS-induced cytokine production is abrogated by a blocking antibody to CD14. • IFN-γ primes human gingival fibroblasts, through up-regulated CD14 expression.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-9969 1879-1506
DOI:	10.1016/j.archoralbio.2015.10.005