Search Results - "Zappulla, David C"

Yeast Telomerase RNA Flexibly Scaffolds Protein Subunits: Results and Repercussions by Zappulla, David C

“…It is said that "hindsight is 20-20", so, given the current year, it is an opportune time to review and learn from experiences studying long noncoding RNAs…”
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A 4-Base-Pair Core-Enclosing Helix in Telomerase RNA Is Essential for Activity and for Binding to the Telomerase Reverse Transcriptase Catalytic Protein Subunit by Mefford, Melissa A., Hass, Evan P., Zappulla, David C.

“…The telomerase ribonucleoprotein (RNP) counters the chromosome end replication problem, completing genome replication to prevent cellular senescence in yeast,…”
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The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae by Hass, Evan P, Zappulla, David C

“…In Saccharomyces cerevisiae and in humans, the telomerase RNA subunit is bound by Ku, a ring-shaped protein heterodimer best known for its function in DNA…”
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Inverse-Folding Design of Yeast Telomerase RNA Increases Activity In Vitro by Lebo, Kevin J, Zappulla, David C

“…Saccharomyces cerevisiae telomerase RNA, TLC1, is an 1157 nt non-coding RNA that functions as both a template for DNA synthesis and a flexible scaffold for…”
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Yeast telomerase RNA: A flexible scaffold for protein subunits by Zappulla, D.C, Cech, T.R

“…In the yeast Saccharomyces cerevisiae, distinct regions of the 1.2-kb telomerase RNA (TLC1) bind to the catalytic subunit Est2p and to accessory proteins. In…”
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Repositioning the Sm-Binding Site in Saccharomyces cerevisiae Telomerase RNA Reveals RNP Organizational Flexibility and Sm-Directed 3'-End Formation by Hass, Evan P, Zappulla, David C

“…Telomerase RNA contains a template for synthesizing telomeric DNA and has been proposed to act as a flexible scaffold for holoenzyme protein subunits in the…”
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Perinuclear localization of chromatin facilitates transcriptional silencing by Sternglanz, Rolf, Andrulis, Erik D, Neiman, Aaron M, Zappulla, David C

“…Transcriptional silencing in Saccharomyces cerevisiae at the HM mating-type loci and telomeres occurs through the formation of a heterochromatin-like…”
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A Flexible Template Boundary Element in the RNA Subunit of Fission Yeast Telomerase by Box, Jessica A., Bunch, Jeremy T., Zappulla, David C., Glynn, Earl F., Baumann, Peter

“…Telomerase adds telomeric repeat sequences to chromosome ends using a short region of its RNA subunit as a template. Telomerase RNA subunits are…”
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Esc1, a Nuclear Periphery Protein Required for Sir4-Based Plasmid Anchoring and Partitioning by Andrulis, Erik D., Zappulla, David C., Ansari, Athar, Perrod, Severine, Laiosa, Catherine V., Gartenberg, Marc R., Sternglanz, Rolf

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Structural Insights into Yeast Telomerase Recruitment to Telomeres by Chen, Hongwen, Xue, Jing, Churikov, Dmitri, Hass, Evan P., Shi, Shaohua, Lemon, Laramie D., Luciano, Pierre, Bertuch, Alison A., Zappulla, David C., Géli, Vincent, Wu, Jian, Lei, Ming

“…Telomerase maintains chromosome ends from humans to yeasts. Recruitment of yeast telomerase to telomeres occurs through its Ku and Est1 subunits via…”
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Control of replication timing by a transcriptional silencer by Zappulla, David C, Sternglanz, Rolf, Leatherwood, Janet

“…Eukaryotic DNA replication starts at many origins. Some origins are used early in S phase, while others are programmed to fire later. In general, late…”
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Physical Connectivity Mapping by Circular Permutation of Human Telomerase RNA Reveals New Regions Critical for Activity and Processivity by Mefford, Melissa A., Zappulla, David C.

“…Telomerase is a specialized ribonucleoprotein complex that extends the 3′ ends of chromosomes to counteract telomere shortening. However, increased telomerase…”
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Refined secondary-structure models of the core of yeast and human telomerase RNAs directed by SHAPE by Niederer, Rachel O, Zappulla, David C

“…Telomerase catalyzes the addition of nucleotides to the ends of chromosomes to complete genomic DNA replication in eukaryotes and is implicated in multiple…”
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Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq by Niederer, Rachel O., Papadopoulos, Nickolas, Zappulla, David C.

“…Telomerase is a ribonucleoprotein that maintains the ends of linear chromosomes in most eukaryotes. Loss of telomerase activity results in shortening of…”
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A second essential function of the Est1-binding arm of yeast telomerase RNA by Lebo, Kevin J, Niederer, Rachel O, Zappulla, David C

“…The enzymatic ribonucleoprotein telomerase maintains telomeres in many eukaryotes, including humans, and plays a central role in aging and cancer…”
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RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP by Mefford, Melissa A, Rafiq, Qundeel, Zappulla, David C

“…Telomerase is a specialized chromosome end‐replicating enzyme required for genome duplication in many eukaryotes. An RNA and reverse transcriptase protein…”
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Refined secondary-structure models of the core of yeast and human telomerase RNAs directed by SHAPE by Niederer, Rachel O, Zappulla, David C

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Stiffened yeast telomerase RNA supports RNP function in vitro and in vivo by Lebo, Kevin J, Zappulla, David C

“…The 1157-nt Saccharomyces cerevisiae telomerase RNA, TLC1, in addition to providing a 16-nt template region for reverse transcription, has been proposed to act…”
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One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors by Andrulis, E.D, Zappulla, D.C, Alexieva-Botcheva, K, Evangelista, C, Sternglanz, R

“…In Saccharomyces cerevisiae, genes located at the telomeres and the HM loci are subject to transcriptional silencing. Here, we report results of screening a…”
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A miniature yeast telomerase RNA functions in vivo and reconstitutes activity in vitro by Cech, Thomas R, Zappulla, David C, Goodrich, Karen

“…The ribonucleoprotein enzyme telomerase synthesizes DNA at the ends of chromosomes. Although the telomerase catalytic protein subunit (TERT) is well conserved,…”
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