Resistance of Ehrlich tumor cells to apoptosis can be due to accumulation of heat shock proteins

Previously we have found that stationary Ehrlich ascites carcinoma (EAC) cells in vivo accumulated heat shock proteins (HSPs) and became resistant to necrotic heath induced by prolonged energy deprivation of hyperthermia. Here we report that apoptotic death induced by nutrient starvation, transient...

Full description

Saved in:

Bibliographic Details
Published in:	FEBS letters Vol. 375; no. 1; pp. 21 - 26
Main Authors:	Gabai, Vladimir L., Zamulaeva, Irina V., Mosin, Alexei F., Makarova, Yulia M., Mosina, Vera A., Budagova, Karina R., Malutina, Yana V., Kabakov, Alexander E.
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 13-11-1995
Subjects:	Adenosine Triphosphate - metabolism Animals Antineoplastic Agents, Phytogenic - pharmacology Apoptosis Apoptosis - drug effects Apoptosis - physiology Carcinoma, Ehrlich Tumor - metabolism Carcinoma, Ehrlich Tumor - pathology Carcinoma, Ehrlich Tumor - physiopathology CCCP, carbonyl cyanide m-chlorophenylhydrazone Cell Cycle - drug effects Cell Cycle - physiology Chromatin - drug effects Chromatin - ultrastructure DMEM, Dulbecco's modified Eagle's minimal essential medium DNA fragmentation DNA, Neoplasm - analysis DNA, Neoplasm - isolation & purification DNA, Neoplasm - metabolism EAC, Ehrlich ascites carcinoma Ehrlich ascites carcinoma Electrophoresis, Agar Gel Heat shock proteins Heat-Shock Proteins - biosynthesis Hot Temperature HSP, heat shock protein Mice Mice, Inbred C57BL Microtubules - drug effects PBS, phosphate-buffered saline Vinblastine - pharmacology EAC, Ehrlich ascites carcinoma CCCP, carbonyl cyanide m-chlorophenylhydrazone PBS, phosphate-buffered saline DNA fragmentation Ehrlich ascites carcinoma DMEM, Dulbecco's modified Eagle's minimal essential medium Heat shock proteins HSP, heat shock protein Apoptosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Previously we have found that stationary Ehrlich ascites carcinoma (EAC) cells in vivo accumulated heat shock proteins (HSPs) and became resistant to necrotic heath induced by prolonged energy deprivation of hyperthermia. Here we report that apoptotic death induced by nutrient starvation, transient ATP depletion, heat shock and a microtubule-disrupting drug, vinblastine, was also suppressed in stationary EAC cells comparing with exponential cells. When exponential (sensitive) cells were subjected to short-term heating with recovery to accumulate inducible form of HSP70, they also became resistant to all of the employed apoptosis-inducing exposures, and an inhibitor of cytosolic protein synthesis, cycloheximide, prevented acquisition of the resistance. It is suggested that in vivo accumulation of HSPs in stationary tumor cells can be endogenous protective device against apoptotic death induced by starvation or some anticancer treatments.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(95)01152-5