Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii

Crude methanol extracts of a marine sponge, Aaptos aaptos , collected from three different localities namely Kapas, Perhentian and Redang Islands, Terengganu, Malaysia, were tested in vitro on a pathogenic Acanthamoeba castellanii (IMR isolate) to examine their anti-amoebic potential. The examinatio...

Full description

Saved in:

Bibliographic Details
Published in:	World journal of microbiology & biotechnology Vol. 28; no. 3; pp. 1237 - 1244
Main Authors:	Nakisah, M. A., Ida Muryany, M. Y., Fatimah, H., Nor Fadilah, R., Zalilawati, M. R., Khamsah, S., Habsah, M.
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-03-2012 Springer Nature B.V
Subjects:	Aaptos Aaptos aaptos Acanthamoeba castellanii Acanthamoeba castellanii - drug effects Acanthamoeba castellanii - growth & development Acanthamoeba castellanii - ultrastructure Amoeba Animals Antimicrobial agents Antiprotozoal Agents - isolation & purification Antiprotozoal Agents - pharmacology Apoptosis Applied Microbiology Bioassays Biochemistry Biomedical and Life Sciences Biotechnology Cytotoxicity Damage Deoxyribonucleic acid DNA DNA damage DNA Damage - drug effects Environmental Engineering/Biotechnology Fluorescence Fluorescence microscopy Genotoxicity Inhibitory Concentration 50 Iodides Life Sciences Malaysia Marine Methanol Methyl alcohol Microbiology Microscopy, Electron, Scanning Microscopy, Fluorescence Morphology Natural products Original Paper Parasitic Sensitivity Tests - methods Porifera - chemistry Scanning electron microscopy Science Sponges Studies Temperature Time Factors Malaysia ISEW, Malaysia Malaysia, Malaya, Terengganu Scanning electron microscopy IC Comet assay Apoptosis
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Crude methanol extracts of a marine sponge, Aaptos aaptos , collected from three different localities namely Kapas, Perhentian and Redang Islands, Terengganu, Malaysia, were tested in vitro on a pathogenic Acanthamoeba castellanii (IMR isolate) to examine their anti-amoebic potential. The examination of anti-Acanthamoebic activity of the extracts was conducted in 24 well plates for 72 h at 30 °C. All extracts possessed anti-amoebic activity with their IC 50 values ranging from 0.615 to 0.876 mg/mL. The effect of the methanol extracts on the surface morphology of A. castellanii was analysed under scanning electron microscopy. The ability of the extracts to disrupt the amoeba cell membrane was indicated by extensive cell’s blebbing, changes in the surface morphology, reduced in cell size and with cystic appearance of extract-treated Acanthamoeba. Number of acanthapodia and food cup was also reduced in this Acanthamoeba . Morphological criteria of apoptosis in Acanthamoeba following treatment with the sponge’s extracts was determined by acridine orange-propidium iodide staining and observed by fluorescence microscopy. By this technique, apoptotic and necrotic cells can be visualized and quantified. The genotoxic potential of the methanol extracts was performed by the alkaline comet assay. All methanol extracts used were significantly induced DNA damage compared to untreated Acanthamoeba by having high percentage of scores 1, 2, and 3 of the DNA damage. Results from cytotoxicity and genotoxicity studies carried out in the present study suggest that all methanol extracts of A. aaptos have anti-amoebic properties against A. castellanii.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0959-3993 1573-0972
DOI:	10.1007/s11274-011-0927-8