Search Results - "Zakhartchenko, V."

Somatic gene editing ameliorates skeletal and cardiac muscle failure in pig and human models of Duchenne muscular dystrophy by Moretti, A., Fonteyne, L., Giesert, F., Hoppmann, P., Meier, A. B., Bozoglu, T., Baehr, A., Schneider, C. M., Sinnecker, D., Klett, K., Fröhlich, T., Rahman, F. Abdel, Haufe, T., Sun, S., Jurisch, V., Kessler, B., Hinkel, R., Dirschinger, R., Martens, E., Jilek, C., Graf, A., Krebs, S., Santamaria, G., Kurome, M., Zakhartchenko, V., Campbell, B., Voelse, K., Wolf, A., Ziegler, T., Reichert, S., Lee, S., Flenkenthaler, F., Dorn, T., Jeremias, I., Blum, H., Dendorfer, A., Schnieke, A., Krause, S., Walter, M. C., Klymiuk, N., Laugwitz, K. L., Wolf, E., Wurst, W., Kupatt, C.

“…Frameshift mutations in the DMD gene, encoding dystrophin, cause Duchenne muscular dystrophy (DMD), leading to terminal muscle and heart failure in patients…”
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Mitochondrial Distribution and Adenosine Triphosphate Content of Bovine Oocytes Before and After In Vitro Maturation: Correlation with Morphological Criteria and Developmental Capacity After In Vitro Fertilization and Culture by STOJKOVIC, Miodrag, MACHADO, Sergio A, STOJKOVIC, Petra, ZAKHARTCHENKO, Valeri, HUTZLER, Peter, GONCALVES, Paolo B, WOLFS, Eckhard

“…In this study, we evaluated mitochondrial distribution and ATP content of individual bovine oocytes before and after in vitro maturation (IVM). Cumulus-oocyte…”
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Cell-Mediated Transgenesis in Rabbits: Chimeric and Nuclear Transfer Animals by Zakhartchenko, V, Flisikowska, T, Li, S, Richter, T, Wieland, H, Durkovic, M, Rottmann, O, Kessler, B, Gungor, T, Brem, G, Kind, A, Wolf, E, Schnieke, A

“…The ability to perform precise genetic engineering such as gene targeting in rabbits would benefit biomedical research by enabling, for example, the generation…”
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Ultra-Structural Alterations in In Vitro Produced Four-Cell Bovine Embryos Following Controlled Slow Freezing or Vitrification by Cavusoglu, T., Popken, J., Guengoer, T., Yilmaz, O., Uyanikgil, Y., Ates, U., Baka, M., Oztas, E., Zakhartchenko, V.

“…Summary Cryopreservation is the process of freezing and preserving cells and tissues at low temperatures. Controlled slow freezing and vitrification have…”
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Effects of serum starvation and re-cloning on the efficiency of nuclear transfer using bovine fetal fibroblasts by Zakhartchenko, V, Durcova-Hills, G, Stojkovic, M, Schernthaner, W, Prelle, K, Steinborn, R, Müller, M, Brem, G, Wolf, E

“…The developmental potential of bovine fetal fibroblasts was evaluated using nuclear transfer. Fibroblasts from a 37-day-old fetus were fused to enucleated…”
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Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer by Alberio, R, Zakhartchenko, V, Motlik, J, Wolf, E

“…Events after fertilisation have been carefully studied in the last decades. However, there are still several questions to be clarified in relation to the…”
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G.P.82 by Walter, M.C, Klymiuk, N, Blutke, A, Graf, A, Krause, S, Wuensch, A, Krebs, S, Kessler, B, Zakhartchenko, V, Kurome, M, Kemter, E, Nagashima, H, Schoser, B, Herbach, N, Blum, H, Wanke, R, Aartsma-Rus, A, Lochmuller, H, Wolf, E

“…Duchenne muscular dystrophy (DMD) is caused by mutations in the X-linked dystrophin (DMD) gene. The absence of dystrophin protein leads to progressive muscle…”
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Intracytoplasmic sperm injection in bovine: Effects of oocyte activation, sperm pretreatment and injection technique by Suttner, R., Zakhartchenko, V., Stojkovic, P., Müller, S., Alberio, R., Medjugorac, I., Brem, G., Wolf, E., Stojkovic, M.

“…Intracytoplasmic sperm injection (ICSI) is a very important technique for treating male subfertility and for basic research. The efficiency of ICSI in bovine…”
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Transgenic technology in farm animals - progress and perspectives by Wolf, Eckhard, Schernthaner, Wolfgang, Zakhartchenko, Valeri, Prelle, Katja, Stojkovic, Miodrag, Brem, Gottfried

“…Current applications of gene transfer in farm animals include the improvement of product quality and quantity, disease resistance, the production of valuable…”
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Nucleolar proteins and ultrastructure in bovine in vivo developed, in vitro produced, and parthenogenetic cleavage-stage embryos by Laurincik, J., Schmoll, F., Mahabir, E., Schneider, H., Stojkovic, M., Zakhartchenko, V., Prelle, K., Hendrixen, P.J.M., Voss, P.L.A.M., Moeszlacher, G.G., Avery, B., Dieleman, S.J., Besenfelder, U., Müller, M., Ochs, R.L., Wolf, E., Schellander, K., Maddox-Hyttel, P.

“…In the present study, ribosomal RNA (rRNA) gene activation, monitored through nucleolus development, was studied by autoradiography following 3H‐uridine…”
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Secretion of interferon-tau by bovine embryos in long-term culture: comparison of in vivo derived, in vitro produced, nuclear transfer and demi-embryos by Stojkovic, M, Büttner, M, Zakhartchenko, V, Riedl, J, Reichenbach, H.-D, Wenigerkind, H, Brem, G, Wolf, E

“…Interferon-tau (IFNτ) is the pregnancy recognition signal of bovine embryos, inhibiting luteolysis. We studied trophoblastic growth and IFNτ secretion of…”
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Activation of ribosomal RNA genes in preimplantation cattle and swine embryos by Hyttel, P, Laurincik, J, Viuff, D, Fair, T, Zakhartchenko, V, Rosenkranz, C, Avery, B, Rath, D, Niemann, H, Thomsen, P.D, Schellander, K, Callesen, H, Wolf, E, Ochs, R.L, Greve, T

“…Transcription of ribosomal RNA (rRNA) genes occurs in the nucleolus resulting in ribosome synthesis. In cattle and swine embryos, functional…”
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Nucleolar protein allocation and ultrastructure in bovine embryos produced by nuclear transfer from granulosa cells by Laurincik, J., Zakhartchenko, V., Stojkovic, M., Brem, G., Wolf, E., Müller, M., Ochs, R.L., Maddox-Hyttel, P.

“…In the present study immunofluorescence confocal laser scanning microscopy, autoradiography following 3H‐uridine incubation and transmission electron…”
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Effect of donor cell age on the efficiency of nuclear transfer in rabbits by Galat, V, Lagutina, I, Mezina, M, Prokofiev, MI, Zakhartchenko, V

“…The ability of rabbit fibroblasts of different ages to be reprogrammed following nuclear transfer (NT) to aged recipient oocytes was evaluated. The rate of NT…”
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Support for the development of bovine embryos in vitro by secretions of bovine trophoblastic vesicles derived in vitro by Stojkovic, M, Zakhartchenko, V, Brem, G, Wolf, E

“…This study evaluated whether trophoblastic tissue derived in vitro secretes factors that support bovine embryonic development in vitro . The embryotrophic…”
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A reliable procedure for differential staining of in vitro produced bovine blastocysts: comparison of tissue culture medium 199 and Ménézo's B2 medium by Stojkovic, M, Büttner, M, Zakhartchenko, V, Brem, G, Wolf, E

“…A reliable double-dye technique has been established for counting the number of inner cell mass and trophectoderm cells of in vitro produced bovine…”
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Effect of donor embryo cell number and cell size on the efficiency of bovine embryo cloning by Zakhartchenko, V, Wolf, E, Palma, G A, Brem, G

“…To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor…”
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Quantitation of retroviral-mediated transfer using luciferase in living and lysed cells by Zakhartchenko, V A, Anderson, W F, Rozenberg, Y

“…We have developed a murine retroviral vector containing an improved luciferase gene for the study of retroviral gene transfer and expression in living or lysed…”
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Epigenetic Marking Correlates with Developmental Potential in Cloned Bovine Preimplantation Embryos by Santos, Fátima, Zakhartchenko, Valeri, Stojkovic, Miodrag, Peters, Antoine, Jenuwein, Thomas, Wolf, Eckhard, Reik, Wolf, Dean, Wendy

“…During differentiation, somatic nuclei acquire highly specialized DNA and chromatin modifications, which are thought to result in cellular memory of the…”
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Effects of high concentrations of hyaluronan in culture medium on development and survival rates of fresh and frozen-thawed bovine embryos produced in vitro by Stojkovic, M, Kolle, S, Peinl, S, Stojkovic, P, Zakhartchenko, V, Thompson, JG, Wenigerkind, H, Reichenbach, HD, Sinowatz, F, Wolf, E

“…Hyaluronic acid (HA) is the main glycosaminoglycan present in follicular, oviductal and uterine fluids. The main functions of HA include dynamic processes that…”
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