Role of diagnostic factors associated with antioxidative status and expression of matrix metalloproteinases (MMPs) in patients with cancer therapy induced ocular disorders
Background: Cancer patients when treated with different chemotherapeutic drugs often develop mild to severe sight threatening diseases during or after chemotherapy. The mechanism involved in the pathogenesis of ocular toxicities is poorly understood. Oxidative stress, inflammation and MMPs (angiogen...
Saved in:
| Published in: | Saudi journal of biological sciences Vol. 25; no. 8; pp. 1725 - 1728 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Riyadh, Saudi Arabia
Saudi Biological Society
2018
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background: Cancer patients when treated with different chemotherapeutic drugs often develop mild to
severe sight threatening diseases during or after chemotherapy. The mechanism involved in the pathogenesis
of ocular toxicities is poorly understood. Oxidative stress, inflammation and MMPs (angiogenic
factor) are involved in the progression of chemotherapy related ocular disorders.
Materials and methods: The concentration of oxidative stress markers such as MDA, NO and levels of different
antioxidant molecules such as SOD, CAT, GSH, GPx, GPr, VIT A, VIT E and VIT C present in the serum
of chemotherapy treated patients (n = 50) and in normal persons (n = 20) were estimated by the direct
spectrophotometric method while the concentration of TNF-a and MMP-9 activity were determined
using human TNF-a and MMP-9 ELISA kits.
Results: The concentration of SOD and CAT (0.356 ± 0.05 lg/dl and 1.26 ± 0.01 lmol/mol of protein) was
significantly lower as compared to that (1.09 ± 0.03 lg/dl and 3.99 ± 0.04 lmol/mol of protein) in controls.
The levels of GPx (0.06 ± 0.01 mmol/dl) in the cancer patients were much lower than those in the controls
(0.78 ± 0.06 mmol/dl). Lower level of GSH (0.96 ± 0.003 lg/dl) in serum of the diseased group was
observed as compared to healthy group (7.26 ± 1.40 lg/dl). The level of Vit A, Vit C and Vit E was lower
in systemic circulation of cancer patients (109.99 ± 6.35 lg/ml, 1.26 ± 0.36 lg/ml and 1.29 ± 0.191 lg/ml)
as compared to control subjects (166.35 ± 14.26 lg/ml, 3.25 ± 0.099 lg/ml and 6.354 ± 2.26 lg/ml)
respectively. The concentration of nitric oxide was significantly higher in the cancer patients
(45.26 ± 6.35 ng/ml) than that in the normal subjects (16.35 ± 3.26 ng/ml). The higher concentration of
MDA (8.65 ± 3.26 nmol/ml) was observed in the patients than normal ones (1.254 ± 0.065 nmol/ml).
The quantity of TNF-a was significantly higher in chemotherapy treated patients (32.68 ± 4.33 pg/ml) as compared to the control group (20.979 ± 1.98 pg/ml). Significantly higher concentration of MMP-9
(40.26 ± 3.26 ng/ml) was observed in the cancer patients than the controls (7.256 ± 1.95 ng/ml).
Conclusion: Lower levels of antioxidant enzymes and non-enzymatic small molecules and higher levels of
oxidative stress and inflammatory clinical parameters such as NO, MDA, TNF-a and MMP-9 may be
involved in the pathogenesis of systemic chemotherapy related ocular complications such as cataract,
glaucoma, blepharitis, retinitis pigmentosa, macular degeneration, pterygium and retinal degeneration. |
|---|---|
| ISSN: | 1319-562X |