High-fat diet effect on periapical lesions and hepatic enzymatic antioxidant in rats

To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats. Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of th...

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Bibliographic Details
Published in:	Life sciences (1973) Vol. 264; p. 118637
Main Authors:	Tibúrcio-Machado, Camilla dos Santos, Lang, Pauline Mastella, Campos, Maria Martha, Wolle, Carlos Frederico Brilhante, Barcelos, Raquel Cristine Silva, Zago, Natália Brezolin, Leite, Carlos Eduardo, Etges, Adriana, Bier, Carlos Alexandre Souza
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Inc 01-01-2021 Elsevier BV
Subjects:	Animals Antioxidants Antioxidants - metabolism Biochemical analysis Blood Blood glucose Body size Body weight Catalase Catalase - metabolism Cholesterol Diet Diet, High-Fat Euthanasia Glucose Glucose tolerance Glucose Tolerance Test Glutathione Glutathione - metabolism High fat diet Inflammation Insulin Insulin Resistance Lipids Lipids - blood Liver - enzymology Male Molars Oxidative Stress Periapical lesion Periapical periodontitis Periodontitis Pulp Radiographs Radiography Rats Rats, Wistar Rodents Sucrose Periapical periodontitis Oxidative stress Periapical lesion High-fat diet
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Summary:	To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats. Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of the mandibular first molars were exposed to develop AP. A glucose tolerance test was performed the week before euthanasia. At the tenth experimental week, the animals were euthanized and the livers were collected to estimate catalase (CAT) and reduced glutathione (GSH) levels. Blood was acquired for biochemical analysis. The size of AP was estimated from radiographs and described as AP size-to-body weight ratio; inflammatory grade of AP was determined by histological analysis. At the end of the experimental period, the rats fed the HFD had 30% less weight (P < 0.0001) and higher blood glucose levels after 30 min of sucrose intake (P < 0.05) than those fed the SD. Animals from the HFD group had lower levels of CAT (P < 0.01), but the same was not observed in the GSH levels. Plasma insulin and total cholesterol were not affected by the diet. The rats fed the HFD presented greater AP than those fed the SD (P < 0.05). However, the local inflammatory infiltrate was similar in both groups. The alterations promoted by the consumption of a HFD were not only observed systemically, but also locally, producing greater AP in rats than a SD.
ISSN:	0024-3205 1879-0631
DOI:	10.1016/j.lfs.2020.118637