Search Results - "ZHANG, YI P."
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ATP regeneration by ATPases for in vitro biotransformation
Published in Biotechnology advances (01-07-2024)“…Adenosine triphosphate (ATP) regeneration is a significant step in both living cells and in vitro biotransformation (ivBT). Rotary motor ATP synthases…”
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Enzymatic Electrosynthesis of Glycine from CO2 and NH3
Published in Angewandte Chemie International Edition (27-03-2023)“…Enzymatic electrosynthesis has gained more and more interest as an emerging green synthesis platform, particularly for the fixation of CO2. However, the…”
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Carrier‐Free Immobilization of Multi‐Enzyme Complex Facilitates In Vitro Synthetic Enzymatic Biosystem for Biomanufacturing
Published in ChemSusChem (22-03-2023)“…A method is developed for carrier‐free immobilization of multi‐enzyme complexes with more than four enzymes by utilization of polypeptide interactions…”
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Stoichiometric Regeneration of ATP by A NAD(P)/CoA‐free and Phosphate‐balanced In Vitro Synthetic Enzymatic Biosystem
Published in ChemCatChem (21-12-2018)“…The regeneration of adenosine triphosphate is crucial for establishing a cost‐effective biosystem in which energy‐dependent processes are involved. Here, an in…”
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Efficient secretory production of large‐size heterologous enzymes in Bacillus subtilis: A secretory partner and directed evolution
Published in Biotechnology and bioengineering (01-10-2020)“…Secretory production of recombinant proteins provides a simple approach to the production and purification of target proteins in the enzyme industry. We…”
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Remodeling of lumbar motor circuitry remote to a thoracic spinal cord injury promotes locomotor recovery
Published in eLife (12-09-2018)“…Retrogradely-transported neurotrophin signaling plays an important role in regulating neural circuit specificity. Here we investigated whether targeted…”
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Construction of an artificial phosphoketolase pathway that efficiently catabolizes multiple carbon sources to acetyl-CoA
Published in PLoS biology (21-09-2023)“…The canonical glycolysis pathway is responsible for converting glucose into 2 molecules of acetyl-coenzyme A (acetyl-CoA) through a cascade of 11 biochemical…”
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Composition and distribution of internal resistance in an enzymatic fuel cell and its dependence on cell design and operating conditions
Published in RSC advances (05-03-2019)“…A variety of sugar-based enzymatic fuel cells (EFCs) are able to completely oxidize fuels catalyzed by enzyme cascades, achieving high energy densities…”
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Building a Thermostable Metabolon for Facilitating Coenzyme Transport and In Vitro Hydrogen Production at Elevated Temperature
Published in ChemSusChem (21-09-2018)“…To facilitate coenzyme transport and in vitro enzymatic hydrogen production, a multi‐enzyme metabolon comprising a miniscaffoldin containing three cohesins, a…”
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10
High‐efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme
Published in Microbial biotechnology (01-03-2021)“…Summary Hyperthermophilic archaea with unique biochemical and physiological characteristics are important organisms for fundamental research of life science…”
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A shriveled rectangular carbon tube with the concave surface for high-performance enzymatic glucose/O 2 biofuel cells
Published in Biosensors & bioelectronics (01-05-2019)“…In this study, a novel carbon tube was prepared by carbonizing a rectangular polypyrrole (RPPy) tube at a high temperature for the construction of enzymatic…”
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Coevolution of both thermostability and activity of polyphosphate glucokinase from Thermobifida fusca YX
Published in Applied and environmental microbiology (01-08-2018)“…Thermostability and specific activity of enzymes are two of the most important properties for industrial biocatalysts. Here we developed a Petri dish-based…”
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A High-Throughput Method for Directed Evolution of NAD(P)+‑Dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues
Published in ACS catalysis (06-12-2019)“…Engineering flavin-free NAD(P)+-dependent dehydrogenases to reduce biomimetic nicotinamide analogues (mNAD+s) is of importance for eliminating the need for…”
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Stoichiometric Regeneration of Biomimetic Nicotinamide Coenzyme Powered by Biomass Sugars via In Vitro Synthetic Enzymatic Biosystems
Published in ChemSusChem (09-10-2024)“…Biomimetic nicotinamide coenzymes, including nicotinamide mononucleotide (NMN+), have been demonstrated as promising low-cost alternatives to nicotinamide…”
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Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms
Published in Nature communications (31-08-2024)“…Biofilm formation enhances bacterial survival and antibiotic tolerance, but the underlying mechanisms are incompletely understood. Here, we show that biofilm…”
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Protein engineering for electrochemical biosensors
Published in Current opinion in biotechnology (01-08-2022)“…The development of electrochemical biosensors has gained tremendous attention. Protein engineering has been applied for enhancing properties of native redox…”
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Construction of Enzyme-Cofactor/Mediator Conjugates for Enhanced in Vitro Bioelectricity Generation
Published in Bioconjugate chemistry (19-12-2018)“…Cofactor-dependent oxidoreduction and electron transfer play an important role in in vitro bioelectricity generation and many other enzyme biocatalysis…”
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ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA
Published in Nature communications (16-04-2024)“…In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform…”
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A Recombinant 12‐His Tagged Pyrococcus furiosus Soluble [NiFe]‐Hydrogenase I Overexpressed in Thermococcus kodakarensis KOD1 Facilitates Hydrogen‐Powered in vitro NADH Regeneration
Published in Biotechnology journal (01-04-2019)“…Soluble hydrogenase I (SHI) from the hyperthermophilic archaeon Pyrococcus furiosus is a heterotetrameric [NiFe] hydrogenase that catalyzes the reversible…”
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An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design
Published in Synthetic and systems biotechnology (01-09-2018)“…Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value…”
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