Inhibition of Intracellular Cathepsin Activities and Suppression of Immune Responses Mediated by Helper T Lymphocyte Type-2 by Peroral or Intraperitoneal Administration of Vitamin B6

We reported that pyridoxal phosphate (PAP), a coenzyme form of vitamin B6, strongly inhibits activities of cathepsin B and weakly inhibits those of cathepsins S, K, and C in vitro. Either intraperitoneal injection or peroral administration of medication doses of vitamin B6 in the diet caused dose-de...

Full description

Saved in:

Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 272; no. 1; pp. 151 - 155
Main Authors:	Katunuma, N., Matsui, A., Endo, K., Hanba, J., Sato, A., Nakano, M., Yuto, Y., Tada, Y., Asao, T., Himeno, K., Maekawa, Y., Inubushi, T.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 27-05-2000
Subjects:	Administration, Oral AIDS/HIV Animals cathepsin B cathepsin B, immune suppression, vitamin B6 cathepsin L cathepsins Cathepsins - antagonists & inhibitors diet dipeptidyl-peptidase I Dose-Response Relationship, Drug enzyme inhibitors Immunoglobulins - biosynthesis Immunosuppressive Agents - administration & dosage Immunosuppressive Agents - pharmacology Injections, Intraperitoneal liver Liver - drug effects Liver - enzymology Male Mice Mice, Inbred BALB C Passive Cutaneous Anaphylaxis - drug effects Protease Inhibitors - administration & dosage Protease Inhibitors - pharmacology Pyridoxal - pharmacology pyridoxal phosphate Pyridoxal Phosphate - pharmacology pyridoxine Pyridoxine - administration & dosage Pyridoxine - pharmacology Rats Rats, Wistar Th2 Cells - drug effects Th2 Cells - immunology cathepsin B, immune suppression, vitamin B6
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We reported that pyridoxal phosphate (PAP), a coenzyme form of vitamin B6, strongly inhibits activities of cathepsin B and weakly inhibits those of cathepsins S, K, and C in vitro. Either intraperitoneal injection or peroral administration of medication doses of vitamin B6 in the diet caused dose-dependent inhibition of hepatic cathepsins B, L, S, and C, and the inhibition was exhibited much more significantly in the case of a high protein diet than in a low protein diet. Administration of vitamin B6 induced the suppression of immune responses against ovalbumin (OVA) mediated by helper T lymphocyte type-2, based on the suppression of antigen processing by cathepsin B inhibition, as in the case of CA-074 administration, a cathepsin B specific inhibitor. Ovalbumin-dependent production of immunoglobulins IgE, IgG1 and interleukin IL-4 was suppressed by administration of medication doses of pyridoxal (PA) or pyridoxine (PI), while the production of IgG2a and interferon (INF)-γ mediated by helper T lymphocyte type 1 was not changed. Administration of medication doses of vitamin B6 caused the inhibition of intracellular cathepsin B activity due to suppression of the functions of helper T lymphocyte type-2.
Bibliography:	http://www.academicpress.com/bbrc http://www.sciencedirect.com/science/journal/0006291X ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.2000.2738