Effect of systemic parathyroid hormone (1-34) and a β-tricalcium phosphate biomaterial on local bone formation in a critical-size rat calvarial defect model

Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 1...

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Published in:	Journal of clinical periodontology Vol. 37; no. 5; pp. 419 - 426
Main Authors:	Yun, Jonathan I., Wikesjö, Ulf ME, Borke, James L., Bisch, Frederick C., Lewis, Jill E., Herold, Robert W., Swiec, Gary D., Wood, Joseph C., McPherson III, James C.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-05-2010
Subjects:	Animals Bone Density Conservation Agents - administration & dosage Bone Density Conservation Agents - pharmacology Bone Matrix - transplantation Bone Regeneration - drug effects Calcium Phosphates - pharmacology Dentistry guided bone regeneration Injections Male parathyroid hormone Parathyroid Hormone - administration & dosage Parathyroid Hormone - pharmacology Random Allocation rat calvaria model Rats Rats, Sprague-Dawley Skull - surgery tissue engineering β-tricalcium phosphate
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Summary:	Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 10.1111/j.1600‐051X.2010.01547.x Objective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation. Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis. Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β‐TCP (22.4±3.0%), β‐TCP (21.3±4.4%), and with the sham‐surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β‐TCP and β‐TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β‐TCP biomaterial. Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β‐TCP biomaterial.
Bibliography:	ark:/67375/WNG-2Z5FH1ZZ-R ArticleID:JCPE1547 istex:FA40B0D3E344D24E7548F6584A7FF690C776C811 The authors report no conflict of interest. The study was funded in its entirety by the USA DENTAC, Fort Gordon, GA. Conflict of interest and source of funding statement ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0303-6979 1600-051X
DOI:	10.1111/j.1600-051X.2010.01547.x