Search Results - "Yuk, Inn H."

Interaction of cell culture process parameters for modulating mAb afucosylation by Nguyen Dang, Anh, Mun, Melissa, Rose, Christopher M., Ahyow, Patrick, Meier, Angela, Sandoval, Wendy, Yuk, Inn H.

“…The extent of afucosylation, which refers to the absence of core fucose on Fc glycans, can correlate positively with the antibody‐dependent cellular…”
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Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases by Zhou, Meixia, Crawford, Yongping, Ng, Domingos, Tung, Jack, Pynn, Abigail F.J., Meier, Angela, Yuk, Inn H., Vijayasankaran, Natarajan, Leach, Kimberly, Joly, John, Snedecor, Bradley, Shen, Amy

“…Large-scale fed-batch cell culture processes of CHO cells are the standard platform for the clinical and commercial production of monoclonal antibodies…”
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FAMS-A Targeted Fatty Acid Mass Spectrometry Method for Monitoring Free Fatty Acids from Polysorbate Hydrolysis by Bathke, Anja, Hoelterhoff, Sina, Wendler, Jan, Yuk, Inn H, Bell, Christian H

“…Polysorbates are the predominant surfactants used to stabilize protein formulations. Unfortunately, polysorbates can undergo hydrolytic degradation, which…”
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Culture temperature modulates aggregation of recombinant antibody in cho cells by Gomez, Natalia, Subramanian, Jayashree, Ouyang, Jun, Nguyen, Mary D.H., Hutchinson, Matthew, Sharma, Vikas K., Lin, Andy A., Yuk, Inn H.

“…During production of therapeutic monoclonal antibodies (mAb), it is highly desirable to remove and control antibody aggregates in the manufacturing process to…”
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Formulation mitigations for particle formation induced by enzymatic hydrolysis of polysorbate 20 in protein-based drug products: insights from a full-factorial longitudinal study by Yuk, Inn H., Koulis, Theo, Doshi, Nidhi, Gregoritza, Kathrin, Hediger, Constanze, Lebouc-Haefliger, Vanessa, Giddings, Jamie, Khan, Tarik A.

“…Hydrolytic degradation of the polysorbate 20 (PS20) surfactant in protein-based liquid formulations releases free fatty acids (FFAs), which can accumulate to…”
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Characterization of Recombinantly-Expressed Hydrolytic Enzymes from Chinese Hamster Ovary Cells: Identification of Host Cell Proteins that Degrade Polysorbate by Kovner, Daniel, Yuk, Inn H, Shen, Amy, Li, Hong, Graf, Tobias, Gupta, Sanjay, Liu, Wenqiang, Tomlinson, Anthony

“…Enzymatic hydrolysis of polysorbate in drug products is a major challenge for the biopharmaceutical industry. Polysorbate hydrolysis caused by host cell…”
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Effect of temperature, pH, dissolved oxygen, and hydrolysate on the formation of triple light chain antibodies in cell culture by Gomez, Natalia, Ouyang, Jun, Nguyen, Mary D. H., Vinson, Abigail R., Lin, Andy A., Yuk, Inn H.

“…THIOMABs are recombinant antibodies with reactive cysteine residues used for forming THIOMAB–drug conjugates (TDCs). We recently reported a new impurity…”
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High-Throughput, Fluorescence-Based Esterase Activity Assay for Assessing Polysorbate Degradation Risk during Biopharmaceutical Development by Bhargava, Adithi C., Mains, Kathryn, Siu, Albert, Gu, Jie, Zarzar, Jonathan, Yi, Li, Yuk, Inn H.

“…Purpose Hydrolytic degradation of polysorbate during 2–8°C storage of monoclonal antibody drug products has been attributed to residual enzymes (e.g.,…”
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Utilizing targeted integration CHO pools to potentially accelerate the GMP manufacturing of monoclonal and bispecific antibodies by Barnard, Gavin C., Zhou, Michelle, Shen, Amy, Yuk, Inn H., Laird, Michael W.

“…Monoclonal antibodies (mAbs) are effective therapeutic agents against many acute infectious diseases including COVID‐19, Ebola, RSV, Clostridium difficile, and…”
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Versatile LC–MS-Based Workflow with Robust 0.1 ppm Sensitivity for Identifying Residual HCPs in Biotherapeutic Products by Yang, Feng, Li, Delia, Kufer, Regina, Cadang, Lance, Zhang, Jennifer, Dai, Lu, Guo, Jia, Wohlrab, Stefanie, Greenwood-Goodwin, Midori, Shen, Amy, Duan, Dana, Li, Hong, Yuk, Inn H

“…Residual host cell proteins (HCPs) in the drug product can affect product quality, stability, and/or safety. In particular, highly active hydrolytic enzymes at…”
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A fast and sensitive high-throughput assay to assess polysorbate-degrading hydrolytic activity in biopharmaceuticals by Gupta, Sanjay K., Graf, Tobias, Edelmann, Franziska T., Seelmann, Helen, Reintinger, Markus, Hillringhaus, Lars, Bergmann, Frank, Wiedmann, Michael, Falkenstein, Roberto, Wegele, Harald, Yuk, Inn H., Leiss, Michael

“…[Display omitted] Hydrolysis of polysorbate in biopharmaceutical products has been ascribed to the enzymatic activity from trace levels of residual host cell…”
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Perfusion cultures of human tumor cells: a scalable production platform for oncolytic adenoviral vectors by Yuk, Inn H. Y., Olsen, Matthew M., Geyer, Scott, Forestell, Sean P.

“…This study demonstrates the novel use of the HeLaS3 human tumor cell line for propagating ONYX‐411, a recombinant oncolytic adenoviral vector. HeLaS3 cells…”
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A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering by Carver, Joseph, Kern, Marie, Ko, Peggy, Greenwood‐Goodwin, Midori, Yu, X. Christopher, Duan, Dana, Tang, Danming, Misaghi, Shahram, Auslaender, Simon, Haley, Ben, Yuk, Inn H., Shen, Amy

“…Chinese hamster ovary (CHO) cell engineering based on CRISPR/Cas9 knockout (KO) technology requires the delivery of guide RNA (gRNA) and Cas9 enzyme for…”
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Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors by Hsu, Wei-Ting, Aulakh, Rigzen P. S., Traul, Donald L., Yuk, Inn H.

“…In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an…”
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Effects of copper on CHO cells: Cellular requirements and product quality considerations by Yuk, Inn H., Russell, Stephen, Tang, Yun, Hsu, Wei-Ting, Mauger, Jacob B., Aulakh, Rigzen P. S., Luo, Jun, Gawlitzek, Martin, Joly, John C.

“…Recent reports highlight the impact of copper on lactate metabolism: CHO cell cultures with higher initial copper levels shift to net lactate consumption and…”
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Investigation of Metal-Catalyzed Antibody Carbonylation With an Improved Protein Carbonylation Assay by Yang, Yi, Mah, Anna, Yuk, Inn H, Grewal, Parbir S, Pynn, Abigail, Cole, Will, Gao, Di, Zhang, Fan, Chen, Jia, Gennaro, Lynn, Schöneich, Christian

“…Protein carbonylation is a posttranslational modification referring to the occurrence of aldehydes and ketones in proteins. The current understanding of how…”
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More similar than different: Host cell protein production using three null CHO cell lines by Yuk, Inn H., Nishihara, Julie, Walker Jr, Donald, Huang, Eric, Gunawan, Feny, Subramanian, Jayashree, Pynn, Abigail F.J., Yu, X.Christopher, Zhu-Shimoni, Judith, Vanderlaan, Martin, Krawitz, Denise C.

“…ABSTRACT To understand the diversity in the cell culture harvest (i.e., feedstock) provided for downstream processing, we compared host cell protein (HCP)…”
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Effects of copper on CHO cells: Insights from gene expression analyses by Yuk, Inn H., Zhang, Jitao David, Ebeling, Martin, Berrera, Marco, Gomez, Natalia, Werz, Silke, Meiringer, Christoph, Shao, Zhixin, Swanberg, Jeffrey C., Lee, Kelvin H., Luo, Jun, Szperalski, Berthold

“…Copper concentration can impact lactate metabolism in Chinese Hamster ovary (CHO) cells. In our previous study, a 20‐fold increase in initial copper…”
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Controlling glycation of recombinant antibody in fed-batch cell cultures by Yuk, Inn H., Zhang, Boyan, Yang, Yi, Dutina, George, Leach, Kimberly D., Vijayasankaran, Natarajan, Shen, Amy Y., Andersen, Dana C., Snedecor, Bradley R., Joly, John C.

“…Protein glycation is a non‐enzymatic glycosylation that can occur to proteins in the human body, and it is implicated in the pathogenesis of multiple chronic…”
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Short‐ and long‐term effects on mAb‐producing CHO cell lines after cryopreservation by Subramanian, Jayashree, Aulakh, Rigzen P. S., Grewal, Parbir S., Sanford, Mark, Pynn, Abigail F. J., Yuk, Inn H.

“…Cryopreservation provides the foundation for research, development, and manufacturing operations in the CHO‐based biopharmaceutical industry. Despite its…”
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