OsATL38 mediates mono-ubiquitination of the 14-3-3 protein OsGF14d and negatively regulates the cold stress response in rice

Abstract One of the major regulatory pathways that permits plants to convert an external stimulus into an internal cellular response within a short period of time is the ubiquitination pathway. In this study, OsATL38 was identified as a low temperature-induced gene that encodes a rice homolog of Ara...

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Published in:	Journal of experimental botany Vol. 73; no. 1; pp. 307 - 323
Main Authors:	Cui, Li Hua, Min, Hye Jo, Yu, Seong Guan, Byun, Mi Young, Oh, Tae Rin, Lee, Andosung, Yang, Hee Woong, Kim, Woo Taek
Format:	Journal Article
Language:	English
Published:	UK Oxford University Press 05-01-2022
Subjects:	14-3-3 Proteins - genetics Cold-Shock Response Gene Expression Regulation, Plant Oryza - genetics Oryza - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Stress, Physiological Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism Ubiquitination Really Interesting New Gene (RING) E3 ubiquitin (Ub) ligase cold stress tolerance mono-ubiquitination OsGF14d 14-3-3 protein family Arabidopsis Tóxicos en Levadura (ATL) family ) rice rice (Oryza sativa)
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Summary:	Abstract One of the major regulatory pathways that permits plants to convert an external stimulus into an internal cellular response within a short period of time is the ubiquitination pathway. In this study, OsATL38 was identified as a low temperature-induced gene that encodes a rice homolog of Arabidopsis Tóxicos en Levadura RING-type E3 ubiquitin (Ub) ligase, which was predominantly localized to the plasma membrane. OsATL38-overexpressing transgenic rice plants exhibited decreased tolerance to cold stress as compared with wild-type rice plants. In contrast, RNAi-mediated OsATL38 knockdown transgenic progeny exhibited markedly increased tolerance to cold stress relative to that of wild-type plants, which indicated a negative role of OsATL38 in response to cold stress. Yeast two-hybrid, in vitro pull-down, and co-immunoprecipitation assays revealed that OsATL38 physically interacted with OsGF14d, a rice 14-3-3 protein. An in vivo target ubiquitination assay indicated that OsGF14d was mono-ubiquitinated by OsATL38. osgf14d knockout mutant plants were more sensitive to cold stress than wild-type rice plants, indicating that OsGF14d is a positive factor in the response to cold stress. These results provide evidence that the RING E3 Ub ligase OsATL38 negatively regulates the cold stress response in rice via mono-ubiquitination of OsGF14d 14-3-3 protein. A RING-type E3 Ub ligase, OsATL38, negatively regulates the cold stress response via mono-ubiquitination of a 14-3-3 protein, OsGF14d, in rice.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-0957 1460-2431
DOI:	10.1093/jxb/erab392