Induction of Expression of Monocyte Interleukin 1 by Hageman Factor (Factor XII)

The results reported here indicate that activated species of Hageman factor (HF, factor XII), a protein that mediates blood clotting, fibrinolysis, and activation of the complement cascade, induce elaboration of interleukin 1 (IL-1) by human monocytes. Augmentation of IL-1 production in mononuclear...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 89; no. 24; pp. 11969 - 11972
Main Authors:	Toossi, Zahra, Sedor, John R., Mettler, Mark A., Everson, Barbara, Young, Tsuguang, Ratnoff, Oscar D.
Format:	Journal Article
Language:	English
Published:	Washington, DC National Academy of Sciences of the United States of America 15-12-1992 National Acad Sciences National Academy of Sciences
Subjects:	Biological and medical sciences Blood Cell culture techniques Cellular biology Cultured cells Cytokines Enzyme Activation Factor XII - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Humans In Vitro Techniques Inflammation Interleukin-1 - chemistry Interleukin-1 - genetics Interleukin-1 - metabolism Interleukin-6 - biosynthesis Interleukins Messenger RNA Molecular and cellular biology Molecules Monocytes Monocytes - metabolism RNA RNA, Messenger - genetics Solubility Thymocytes Human Cell culture Monocyte Pathophysiology Cytokine Inflammation Activation Gene expression Factor XII Mechanism Interleukin 1 Lipopolysaccharide Coagulation factor
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Summary:	The results reported here indicate that activated species of Hageman factor (HF, factor XII), a protein that mediates blood clotting, fibrinolysis, and activation of the complement cascade, induce elaboration of interleukin 1 (IL-1) by human monocytes. Augmentation of IL-1 production in mononuclear cell cultures was observed when HF was present along with lipopolysaccharide (LPS) but was not observed with HF alone. Furthermore, antiserum to HF abrogated the enhancement of IL-1 in cultures containing HF and LPS. Total IL-1 activity, which represents secreted and cell-associated IL-1, was enhanced in LPS-stimulated mononuclear cultures by HF. In the absence of LPS, the initial activation product of HF, HFa, which contains the serine protease enzyme activity and the surface-binding domains of the protein, induced IL-1β protein and mRNA. In the presence of LPS, the enzymatic moiety (HFf), which is also contained in HF and HFa, amplified IL-1 production. Induction and amplification of monocyte IL-1 by HF provides further evidence for establishing a role for HF in the acute-phase reaction and the cellular immune response.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.89.24.11969